FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281368

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281368
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1225902
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAGCATGGGATGACAGCCTGCGTGTTTGGGTGTTCGG49090.40043983939988675No Hit
GTAATACACGGCCGTGTCCTCGGATGTCAGGCTGCTCAGCTCCATGTAGG29800.2430863152193242No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC27630.22538506340637343No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT27110.2211432887783852No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC25300.20637865016942628No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT23710.19340860851846234No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT23700.19332703592946254No Hit
CTATTACTACTACGCTATGGACGTCTGGGGCCAAGGGACCACGGTCACCG22760.18565921256348386No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA22740.18549606738548433No Hit
GTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG22370.18247788159249273No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC22260.1815805831134952No Hit
GTAGTAATAGAGGCGTGTAACCACTCCAAACCCCCTCTGGTCTGCCGCAC20420.16657122673753694No Hit
CCTCTATTACTACTACGCTATGGACGTCTGGGGCCAAGGGACCACGGTCA19640.16020856479555462No Hit
TAATAGAGGCGTGTAACCACTCCAAACCCCCTCTGGTCTGCCGCACAGTA18790.15327489473057387No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC18400.15009356375958274No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA18380.1499304185815832No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT17900.14601493430959409No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG17280.14095743379160813No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG17010.13875497388861427No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA16870.13761295764261744No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA16840.13736823987561814No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCTC16470.1343500540826265No Hit
TTATTACTGTGCAGCATGGGATGACAGCCTGCGTGTTTGGGTGTTCGGCG16060.13100557793363582No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT16030.1307608601666365No Hit
GACTTAGGACGGTAAGTATGGTCCCTGCGCCGAACACCCAAACACGCAGG15820.12904783579764126No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA15780.12872154544164216No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG15690.1279873921406442No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG15440.12594807741564987No Hit
GTCTGGGCCTGAGGTGAAGAAGCCTGGGACCTCAGTGAAGGTCTCCTGCA14760.12040114136366528No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA14730.12015642359666596No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA14660.11958541547366756No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT14460.11795396369367209No Hit
GTACATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCT14440.11779081851567254No Hit
GTTCCTCACTATGGACTGGATTTGGAGGATCCTCTTCTTGGTGGGAGCAG14420.117627673337673No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14330.11689352003667504No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG14180.11566993120167843No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT14040.11452791495568161No Hit
GGTACCAGCGGCTCCCAGGAACGGCCCCCAAACTCCTCGTCTATGGGAAC14040.11452791495568161No Hit
GTGTATTACTGTGCGGCAGACCAGAGGGGGTTTGGAGTGGTTACACGCCT13500.11012299514969386No Hit
TTACTGTGCAGCATGGGATGACAGCCTGCGTGTTTGGGTGTTCGGCGCAG12790.10433134133070997No Hit
CCCAGGAACGGCCCCCAAACTCCTCGTCTATGGGAACAATCAGCGGCCCT12620.10294460731771382No Hit
GTATGGTCCCTGCGCCGAACACCCAAACACGCAGGCTGTCATCCCATGCT12600.10278146213971427No Hit
GCATGGGATGACAGCCTGCGTGTTTGGGTGTTCGGCGCAGGGACCATACT12580.10261831696171471No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12550.10237359919471539No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12520.10212888142771609No Hit
GCCTACATGGAGCTGAGCAGCCTGACATCCGAGGACACGGCCGTGTATTA12500.10196573624971653No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC14550.050.066134
ATCAACG14800.048.7350853
TATCAAC15850.047.3307152
GTATCAA17000.045.84121
CAACGCA16550.044.0194475
AACGCAG17100.042.5461586
CGCAGAG18750.038.800518
ACGCAGA18900.038.492577
GAATACG6000.037.2112739
GTACATG28950.037.138031
GTGCTAG7300.036.5729521
GGTATCA7800.034.2285351
CGTAATA900.003719635332.0594752
TATTGAG17150.031.1235245
ACATGGG32450.030.8960883
GCAGAGT23650.030.7577329
TGCTAGA9850.029.2929232
TCTACAC13200.028.4140283
CATGGGG15800.027.3892294
TAGTACC2705.8826117E-926.7129524
Produced by FastQC (version 0.11.5)