FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281373

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281373
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1786854
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC47000.26303212237821333No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCGATGATCATTA37340.20897062658728696No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCAG34280.19184555649202453No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCG32390.18126830731553895No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC32350.18104445019011067No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32120.17975727171889813No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT27430.15351002376243386No Hit
TTATGATACCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCA26710.14948059550472506No Hit
GTATCATAATAGATGACCAGGACAGGGGCCTGGCCTGGCTTCTGCTGATA24820.13890334632823947No Hit
CCTATGTGCTGACTCAGCCACCCTCAGTGTCAGTGGCCCCAGGAAAGACG24200.13543356088410133No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA24140.13509777519595895No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT22130.12384895464318854No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC22070.12351316895504612No Hit
GTCCTGGTCATCTATTATGATACCGACCGGCCCTCAGGGATCCCTGAGCG21950.12284159757876133No Hit
GTAATACATGGCGGTGTCCGAGGCCTTCAGGCTGGTCCACTGCAAGTAGG21250.11892409788376665No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA20220.1131597769039888No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT19920.1114808484632768No Hit
GGTATCAGCAGAAGCCAGGCCAGGCCCCTGTCCTGGTCATCTATTATGAT19840.11103313421242027No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC19310.10806702730049574No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19160.10722756308013974No Hit
ATACTAATGATCATCGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTA19030.10650002742249787No Hit
ATGTATTACTGTGCGAGGGGAACGTGTAGCGCAGCAGCTTGTGATGCATC18940.10599634889028427No Hit
GGCTCAGTGACCTCCTATGTGCTGACTCAGCCACCCTCAGTGTCAGTGGC18880.10566056320214187No Hit
GTACATGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCA18020.10084763500543413No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17990.10067974216136293No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA17980.10062377788000586No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA35450.076.6075741
ATCAACG46000.057.134613
TCAACGC46300.056.764414
CAACGCA47500.055.481955
AACGCAG49200.053.5648886
TATCAAC51450.052.05922
CGCAGAG55050.047.871378
ACGCAGA57500.045.706417
GCAGAGT66300.039.7483989
GTACATG56750.033.1299861
ACATGGG56900.030.4978333
CTAGGTC31350.029.628963
CAGAGTA66000.029.3467799
CCTAGGT31950.028.6202072
CATGGGA26600.027.0696474
TACATGG67100.027.0406992
TAGGTCA35650.026.257184
GTAATAC16600.023.8671841
TCCTAGG41900.022.8656831
TGGGAGT18150.022.6132226
Produced by FastQC (version 0.11.5)