FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281374

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281374
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1786854
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC42140.23583348163867895No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCGATGATCATTA33040.18490598560374827No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCAG30720.1719222723289088No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29680.16610198706777385No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC28840.16140098743378026No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCG28320.1584908448032128No Hit
TTATGATACCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCA23370.1307885255314648No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT23330.13056466840603653No Hit
GTATCATAATAGATGACCAGGACAGGGGCCTGGCCTGGCTTCTGCTGATA22480.12580770449068587No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA21240.1188681336024096No Hit
CCTATGTGCTGACTCAGCCACCCTCAGTGTCAGTGGCCCCAGGAAAGACG21180.1185323479142672No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT21020.11763691941255414No Hit
GTAATACATGGCGGTGTCCGAGGCCTTCAGGCTGGTCCACTGCAAGTAGG20830.11657359806676987No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC19760.11058541996156372No Hit
GTCCTGGTCATCTATTATGATACCGACCGGCCCTCAGGGATCCCTGAGCG19250.10773124161235334No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA34700.080.917521
TCAACGC43250.064.095964
ATCAACG43500.063.396543
CAACGCA46450.059.6803055
TATCAAC46700.059.0524522
AACGCAG48200.057.513496
CGCAGAG54400.051.0910078
ACGCAGA54850.050.5405737
GCAGAGT61250.045.0156679
CTAGTAC2901.2732926E-1129.7947183
GTACATG52300.029.3214441
ACATGGG53150.027.7719673
GTAATAC18000.027.1984311
TATACCG2156.5078893E-726.7921525
CAGAGTA59250.026.4873879
ATACCGT3006.366463E-1026.401436
GACCGTT23750.025.7698177
ACCGTTC24500.024.9809478
CTAGGTC31450.024.7263013
CCTAGGT31250.024.4237272
Produced by FastQC (version 0.11.5)