FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281376

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281376
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1924964
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28390.1474832776093475No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT22030.11444369868735207No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16550.077.868951
GTATCAA39550.076.274061
TCAACGC47000.064.0356144
ATCAACG46950.063.7970923
TATCAAC51500.058.440272
CAACGCA52850.057.0837065
AACGCAG54250.055.345146
ACGCAGA62500.047.693977
CGCAGAG62750.047.2744678
GCAGAGT70500.041.764739
GTACATG59100.028.1406291
TACATGG60000.027.0006622
ACATGGG60400.026.2258113
GTATTAG8750.025.5072081
CAGAGTA67500.023.7835439
TATATAC11000.020.9459693
GTATATA11050.020.8495661
CATGGGG40650.020.1923774
TATACTG10550.019.7919545
GTAGACG12700.019.2760624
Produced by FastQC (version 0.11.5)