FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281377

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281377
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2642173
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCAGGTCTCAGGCTGTTCATGTGAAGATAGA73920.279769719848019No Hit
GTGTATTACTGTGCAAAGTCTAAGGGTTTGGAGTGGTTCACAGTCGTCGG58710.22220346661630408No Hit
GCCCTACACTGGCTCCGCCAGGCTCCAGGCAAGGGGCTAGAGTGGCTGGC54070.20464216385528122No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38590.146054024471524No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT36040.13640287748001362No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT33600.1271680544763723No Hit
CCCTTAGACTTTGCACAGTAATACACAGCCGTGTCCTCAGGTCTCAGGCT33150.12546491088963516No Hit
GTCTAAGGGTTTGGAGTGGTTCACAGTCGTCGGATACTACTTTGACTACT31180.11800892674325263No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC30560.11566237335708146No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA30430.11517035409869074No Hit
TTTAGGAGGTGTCCAGTGTCAGGTGCAACTGGTGGAGTCCGGGGGAGGCT30380.1149811159223866No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC29520.11172621928995566No Hit
ACTGTGAACCACTCCAAACCCTTAGACTTTGCACAGTAATACACAGCCGT28610.10828208448122056No Hit
GTTCACAGTCGTCGGATACTACTTTGACTACTGGGGCCAGGGAACCCTGG27580.10438377804935559No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT26690.10101533851114214No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA50600.077.3017961
GGTATCA21100.068.2791061
TCAACGC57500.066.618524
ATCAACG58450.065.658943
CAACGCA61500.062.2844355
AACGCAG63450.060.3691146
TATCAAC65050.059.550612
CGCAGAG74650.051.3107538
ACGCAGA81300.047.202317
GCAGAGT86650.044.2048239
GTACATG69900.031.0191331
ACATGGG68200.030.828313
TACATGG70300.030.1122512
CATGGGA35100.027.2832284
CAGCGCT13750.024.610579
CAGCCGT43550.023.8067939
TACACGG18150.023.8017796
GTACACG17900.023.3301755
CATGGGG42350.022.1024884
ACAGCCG76500.022.023238
Produced by FastQC (version 0.11.5)