FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences823204
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAGGGCCACTCCAAAAATCGTAACCACCGCTGTGGGCTCTCGCACAG42220.5128740871035612No Hit
GTAATACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTAGA34290.41654316548510456No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT31420.38167938931297707No Hit
GTCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTA30840.3746337481353346No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23330.2834048425420674No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCA22830.2773310139406514No Hit
ACTATATCCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATG22570.2741726230679151No Hit
GCCCTAGGTACTACTACTATTACGGTATGGACGTCTGGGGCCAAGGGACC21840.2653048333098479No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18700.22716118969295582No Hit
GTGTATTACTGTGCGAGAGCCCACAGCGGTGGTTACGATTTTTGGAGTGG18500.22473165825238947No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA17640.21428467305795404No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAA17350.21076185246913282No Hit
CCATGTAGACTGTGCTCGTGGACGTGTCCCTGGTCATGGTGACTCTGCCC17120.20796789131248147No Hit
TTATTACTGCATGCAAGCTCTAGAAACTTGGACGTTCGGCCAAGGGACCA17070.2073605084523399No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16410.19934305469847086No Hit
CATGTAGACTGTGCTCGTGGACGTGTCCCTGGTCATGGTGACTCTGCCCT16300.19800681240615936No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16170.1964276169697912No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15970.19399808552922482No Hit
CCGTAATAGTAGTAGTACCTAGGGCCACTCCAAAAATCGTAACCACCGCT15940.19363365581313988No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15380.18683096777955402No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15240.18513029577115758No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC15110.18355110033478944No Hit
CTATTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCT14700.17857056088162837No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC14690.17844908430960005No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14300.17371149800049562No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCT13800.1676376693990797No Hit
CTACAGAAGCCTCTGAGAGGAAAGTTCTTCACCATGGACTGGACCTGGAG13370.162414176801862No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12980.15767659049275756No Hit
GATCTATATGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTTCAGTG12570.1526960510395965No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG12490.15172423846336994No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12410.1507524258871434No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGT11960.14528598014586908No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT11920.14480007385775578No Hit
CTCCCAGGTGCAGCTGGTGCAGTCTGGGGCTGAGGTGAAGAAGCCTGGGG11900.14455712071369914No Hit
TCCAACAACCACATCCCTTCTCTACAGAAGCCTCTGAGAGGAAAGTTCTT11810.1434638315654443No Hit
GTTTATTACTGCATGCAAGCTCTAGAAACTTGGACGTTCGGCCAAGGGAC11620.14115577669690624No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC11590.14079134698082127No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG11510.13981953440459471No Hit
GGATACACCTTCACCAGCTACTATATCCACTGGGTGCGACAGGCCCCTGG11470.13933362811648145No Hit
CTCTACAGCTCCTGATCTATATGGGTTCTAATCGGGCCTCCGGGGTCCCT11160.13556785438360358No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT11100.13483899495143367No Hit
ATATAGATCAGGAGCTGTAGAGACTGCCCTGGCTTCTGCAGGTACCAATC10990.13350275265912218No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA10670.12961550235421598No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCCCTTGGCCGAACGTC10610.12888664292204605No Hit
CTACTATATCCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGA10520.1277933537737912No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10500.12755040062973455No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCC10080.12244838460454517No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC9960.12099066574020535No Hit
GTAACGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCT9960.12099066574020535No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA9950.12086918916817703No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGAATATTCAACCCTAGTGGTGGT9910.12038328288006374No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9880.1200188531639788No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9770.11868261087166727No Hit
CTATATGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTTCAGTGGCA9730.11819670458355401No Hit
ATACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTAGACTG9550.11601012628704428No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9550.11601012628704428No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT9340.11345911827444959No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG9260.11248730569822303No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG9170.11139401654996818No Hit
ATATGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTTCAGTGGCAGT9140.11102958683388321No Hit
CAGTAATACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTA8920.10835710224926022No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA8840.10738528967303367No Hit
GTATTACTGTGCGAGAGCCCACAGCGGTGGTTACGATTTTTGGAGTGGCC8820.10714233652897702No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT8780.10665643024086376No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGT8750.10629200052477879No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8730.10604904738072216No Hit
CTATATCCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGG8730.10604904738072216No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTA8700.1056846176646372No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA8660.10519871137652392No Hit
CTACAGCTCCTGATCTATATGGGTTCTAATCGGGCCTCCGGGGTCCCTGA8660.10519871137652392No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA8530.10361951594015578No Hit
GTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGCCTCC8510.10337656279609915No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA8510.10337656279609915No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCATATAGATCAGGAGCTGTA8480.10301213308001418No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8480.10301213308001418No Hit
GGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAATATTCAAC8410.10216179707581596No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT8360.10155441421567436No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8360.10155441421567436No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGT8360.10155441421567436No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG8350.10143293764364604No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG8330.10118998449958941No Hit
GTAGTACCTAGGGCCACTCCAAAAATCGTAACCACCGCTGTGGGCTCTCG8280.10058260163944782No Hit
AGGTACTACTACTATTACGGTATGGACGTCTGGGGCCAAGGGACCACGGT8250.10021817192336285No Hit
CTCCTGCATAGTAACGGATACAACTATTTGGATTGGTACCTGCAGAAGCC8240.10009669535133453No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)