FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences823204
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAGGGCCACTCCAAAAATCGTAACCACCGCTGTGGGCTCTCGCACAG43860.5327962449162055No Hit
GTAATACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTAGA35730.4340357918571824No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT30500.37050354468637176No Hit
GTCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTA23530.2858343739826337No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCA21830.2651833567378195No Hit
ACTATATCCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATG20910.2540075121112142No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20140.2446538160650337No Hit
GCCCTAGGTACTACTACTATTACGGTATGGACGTCTGGGGCCAAGGGACC19280.23420683087059826No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT18570.22558199425658765No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17330.21051889932507617No Hit
CCATGTAGACTGTGCTCGTGGACGTGTCCCTGGTCATGGTGACTCTGCCC17330.21051889932507617No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAA17210.20906118046073635No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT16800.20408064100757528No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA16660.20237996899917882No Hit
CATGTAGACTGTGCTCGTGGACGTGTCCCTGGTCATGGTGACTCTGCCCT16190.19667057011384784No Hit
TTATTACTGCATGCAAGCTCTAGAAACTTGGACGTTCGGCCAAGGGACCA16040.19484842153342305No Hit
CCGTAATAGTAGTAGTACCTAGGGCCACTCCAAAAATCGTAACCACCGCT15850.192540366664885No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14570.17699136544526023No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14560.1768698888732319No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC14240.17298263856832571No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14230.1728611619962974No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA13170.1599846453612956No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13160.15986316878926732No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC12590.15293900418365314No Hit
GATCTATATGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTTCAGTG12510.15196719160742658No Hit
CTATTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCT12420.1508739024591717No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCT12230.14856584759063365No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC12000.14577188643398234No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT11890.14443564414167084No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG11620.14115577669690624No Hit
CTCCCAGGTGCAGCTGGTGCAGTCTGGGGCTGAGGTGAAGAAGCCTGGGG11570.14054839383676465No Hit
GGATACACCTTCACCAGCTACTATATCCACTGGGTGCGACAGGCCCCTGG11410.13860476868431154No Hit
GTTTATTACTGCATGCAAGCTCTAGAAACTTGGACGTTCGGCCAAGGGAC11270.13690409667591508No Hit
CTCTACAGCTCCTGATCTATATGGGTTCTAATCGGGCCTCCGGGGTCCCT11100.13483899495143367No Hit
CTACAGAAGCCTCTGAGAGGAAAGTTCTTCACCATGGACTGGACCTGGAG11080.13459604180737703No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG10820.13143765093464074No Hit
ATATAGATCAGGAGCTGTAGAGACTGCCCTGGCTTCTGCAGGTACCAATC10800.1311946977905841No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT10750.1305873149304425No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCCCTTGGCCGAACGTC10670.12961550235421598No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGT10510.12767187720176287No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG10470.1271859709136496No Hit
GTGTATTACTGTGCGAGAGCCCACAGCGGTGGTTACGATTTTTGGAGTGG10470.1271859709136496No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10420.126578588053508No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG10120.12293429089265843No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA9970.12111214231223366No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC9630.11698193886327084No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTA9590.11649603257515755No Hit
ATACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTAGACTG9570.11625307943110093No Hit
CAGTAATACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTA9530.11576717314298764No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA9500.1154027434269027No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCC9410.11430945427864782No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT9340.11345911827444959No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG9290.112851735414308No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA9200.11175844626605312No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGAATATTCAACCCTAGTGGTGGT9140.11102958683388321No Hit
CTATATGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTTCAGTGGCA9140.11102958683388321No Hit
TCCAACAACCACATCCCTTCTCTACAGAAGCCTCTGAGAGGAAAGTTCTT9120.1107866336898266No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA9110.11066515711779827No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT8960.10884300853737348No Hit
CTACTATATCCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGA8930.10847857882128852No Hit
GTAACGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCT8890.10799267253317525No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8810.1070208599569487No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG8800.10689938338492039No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8730.10604904738072216No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT8700.1056846176646372No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC8700.1056846176646372No Hit
ATATGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTTCAGTGGCAGT8670.10532018794855225No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG8510.10337656279609915No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGT8440.10252622679190092No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA8390.10191884393175932No Hit
GGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAATATTCAAC8350.10143293764364604No Hit
GTCCAGGGGCCTGTCGCACCCAGTGGATATAGTAGCTGGTGAAGGTGTAT8320.10106850792756109No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8300.10082555478350445No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT8240.10009669535133453No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)