FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281383

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281383
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences892197
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT33610.37671052469353744No Hit
GTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCT32890.36864055808302426No Hit
CTATTATTGCATGCAATCTCTACAAACTTGGACGTTCGGCCAGGGGACCA32610.36550223773449136No Hit
CAATAATAGACCCCAACATCCTCAGCCTCCACTCTGCTGATCTTCAGTGT26170.2933208697182349No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24400.27348220180072336No Hit
ACCTAGGGCCACTCCAAAAATCGTAGCCACCGCTGTGGGCTCTCGCACAG24350.27292178745277107No Hit
GTAATAAATGGCCGTGTCTTCAGATCTCAGGCTCCTCAGTTCCAGGTAGA21460.24052983814112802No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19870.2227086618762448No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17030.19087712691255407No Hit
ATTTATTACTGTGCGAGAGCCCACAGCGGTGGCTACGATTTTTGGAGTGG16900.1894200496078781No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15770.17675468534415606No Hit
ATCCATTACTATGCAGGAGGCTCTCACTAGACCTGCAGGAGATGGAGGCC15650.17540969090907055No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15260.17103845899504255No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15150.1698055474295475No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAAATTTCCTGCA14450.1619597465582153No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG13930.15613143733951135No Hit
GCCCTAGGTACTCCTACTACTACGGTATGGACGTCTGGGGCCAAGGGACC13660.15310519986056892No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT13580.15220853690384523No Hit
GATTTTACACTGAAGATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTCTA13570.15209645403425476No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA13140.14727689064186497No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCT12970.14537148185882715No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12600.14122441568398011No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTGAGAGCCTCCTG12220.13696526663954262No Hit
CTATTTGTACTGGGTGCGACAGGCCCCGGGGCGAGGGCTTGAGTGGATGG12060.13517194072609526No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG11810.13236986898633374No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT11740.13158528889920051No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCCTGGCCGAACGTC11440.1282228028114867No Hit
ATAATAGACCCCAACATCCTCAGCCTCCACTCTGCTGATCTTCAGTGTAA11400.12777447133312486No Hit
GCATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGC11340.1271019741155821No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11240.1259811454196775No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA11200.12553281394131566No Hit
CTACAAACTTGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAACGAAC10970.12295490794073506No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC10520.11791117880916434No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC10470.11735076446121205No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10280.1152211899389933No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA10270.11510910706940282No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC10260.11499702419981238No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10160.11387619550390776No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA10060.11275536680800317No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA10060.11275536680800317No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC9990.11197078672086994No Hit
GGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACA9930.1112982895033272No Hit
GTCTACCTGGAACTGAGGAGCCTGAGATCTGAAGACACGGCCATTTATTA9920.11118620663373671No Hit
GTCTATTATTGCATGCAATCTCTACAAACTTGGACGTTCGGCCAGGGGAC9660.10827205202438475No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9600.10759955480684198No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA9560.10715122332848014No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT9440.10580622889339462No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG9420.1055820631542137No Hit
GTATCAACGCAGAGTACATGGGGATCAGGACTCCTCAGTTCACCTTCTCA9040.1013229141097762No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8950.10031416828346205No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)