FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281384

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281384
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences892197
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT32250.3614672544292348No Hit
CTATTATTGCATGCAATCTCTACAAACTTGGACGTTCGGCCAGGGGACCA30840.3456635698169799No Hit
GTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCT30740.3445427411210753No Hit
ACCTAGGGCCACTCCAAAAATCGTAGCCACCGCTGTGGGCTCTCGCACAG26100.2925362896311017No Hit
CAATAATAGACCCCAACATCCTCAGCCTCCACTCTGCTGATCTTCAGTGT25930.2906308808480638No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22560.25285895379607864No Hit
GTAATAAATGGCCGTGTCTTCAGATCTCAGGCTCCTCAGTTCCAGGTAGA21610.24221108118498494No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18540.2078016402207136No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15770.17675468534415606No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15300.1714867904734044No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14880.16677930995060508No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14670.1644255696892054No Hit
ATCCATTACTATGCAGGAGGCTCTCACTAGACCTGCAGGAGATGGAGGCC14610.16375307247166265No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13830.15501060864360675No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAAATTTCCTGCA13520.15153603968630247No Hit
GATTTTACACTGAAGATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTCTA13460.15086354246875971No Hit
GCCCTAGGTACTCCTACTACTACGGTATGGACGTCTGGGGCCAAGGGACC13220.14817355359858866No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT12700.14234524437988472No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA12650.1417848300319324No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCT12560.14077608420561827No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11960.13405111203019066No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG11800.1322577861167433No Hit
CTATTTGTACTGGGTGCGACAGGCCCCGGGGCGAGGGCTTGAGTGGATGG11780.13203362037756236No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG11740.13158528889920051No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTGAGAGCCTCCTG11480.12867113428984855No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCCTGGCCGAACGTC10990.12317907367991598No Hit
CTACAAACTTGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAACGAAC10770.12071325054892586No Hit
ATAATAGACCCCAACATCCTCAGCCTCCACTCTGCTGATCTTCAGTGTAA10710.12004075333138309No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC10680.1197045047226117No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10470.11735076446121205No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA10450.11712659872203113No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC10230.114660775591041No Hit
GCATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGC10110.11331578115595546No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA9940.11141037237291763No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA9900.11096204089455579No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA9810.10995329506824165No Hit
ATTTATTACTGTGCGAGAGCCCACAGCGGTGGCTACGATTTTTGGAGTGG9760.10939288072028935No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9590.10748747193725153No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC9450.10591831176298508No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC9430.10569414602380416No Hit
GGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACA9390.10524581454544232No Hit
GTCTATTATTGCATGCAATCTCTACAAACTTGGACGTTCGGCCAGGGGAC9210.10322832289281403No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC9110.10210749419690944No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9030.10121083124018575No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11850.060.8992541
ATCAACG12500.056.53653
TCAACGC12500.056.5333184
GGTATCA5050.055.7318651
CAACGCA13450.053.088325
AACGCAG14400.049.5163656
GTATACG450.00981642648.1104131
TACGTCG450.00983826148.083365
CTACGTC605.078474E-448.0725484
CGCAGAG16000.044.5647288
CGTACTG657.5259135E-444.384645
CAGCGCT4000.039.6130949
ACGCAGA20100.035.8327377
GGCAGCG4900.035.2769667
AACCGCG850.00283184733.8935557
GTACGGA850.00283184733.8935556
GAATACG1301.7308997E-533.2417539
GTACATG23550.032.482191
TACATGG23600.032.084122
ACATGGG23050.031.9111213
Produced by FastQC (version 0.11.5)