FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281385

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281385
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences678605
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATATACACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGG34640.5104589562411123No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT20100.29619587241473316No Hit
GTAATAAAGGGCCGTGTCCTCAGATCTCAGGCTGCGCAGTTCCATGTAAA19590.2886804547564489No Hit
GTAATGGATATAACTATTTGGATTGGTACTTGCAGAAGCCAGGGCATTCT19500.28735420458145755No Hit
ACCTAGGGCCAGTCCAAAAATCGTAACCACCGCTGTGGGCTCTCGCACAG18720.2758600363981992No Hit
GTCTGGGACTGAGGTGAAGAAGCCTGGGGCCTCAGTGAGGATTTCCTGCA14850.2188312788735715No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12870.189653775023762No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12600.18567502449878795No Hit
GGCCCTAGGTACTACTACTATTACGGTTTGGACGTCTGGGGCCAAGGGAC11840.17447557857663884No Hit
ACTATATACACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATG10990.16194988247949837No Hit
GTGTATATAGTAACTGGTGAAGGTGTATCCAGATGCCTTGCAGGAAATCC10780.15885529873785192No Hit
CTTTATTACTGTGCGAGAGCCCACAGCGGTGGTTACGATTTTTGGACTGG10640.15679224291008761No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA10400.15325557577677734No Hit
CCTAGGGCCAGTCCAAAAATCGTAACCACCGCTGTGGGCTCTCGCACAGT10310.151929325601786No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10260.15119251994901306No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC9280.13675112915466286No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC9280.13675112915466286No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9160.13498279558800774No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT9060.1335091842824618No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT9030.13306710089079804No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC9010.13277237862968885No Hit
GGATATAACTATTTGGATTGGTACTTGCAGAAGCCAGGGCATTCTCCGCA8800.12967779488804237No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8660.12761473906027807No Hit
GTCCCCACTCTCCCTGTCCGTCACCCCTGGAGAGCCGGCCTCTATTTCCT8460.1246675164491862No Hit
TCCAACAACCACATCCCTTCTCTACAGAAGCCTCTGAGAGGAAAGTTCTT8360.12319390514364027No Hit
GTCTACGCACAGAAGTTCCAGGGCAGAGTCACCGTGACCAGGGACACGTC8340.12289918288253107No Hit
GATTTTACACTGAGAATCAGTAGAGTGGAGGCTGAGGATGTTGGGGTTTA8240.12142557157698514No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA8230.12127821044643054No Hit
CCCCTGGAGAGCCGGCCTCTATTTCCTGCAGGTCAAGTCAGAGCCTCCTG8220.12113084931587596No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8060.11877307122700245No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG7850.11567848748535599No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT7810.11508904296313761No Hit
CTCCCAGGTGCACCTGGTGCAGTCTGGGACTGAGGTGAAGAAGCCTGGGG7800.11494168183258302No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG7590.11184709809093656No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT7230.10654209739097119No Hit
CTACAGAAGCCTCTGAGAGGAAAGTTCTTCACCATGGACTGGACCTGGAG7190.10595265286875281No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG6920.10197390234377877No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCT6880.10138445782156041No Hit
CCGTAATAGTAGTAGTACCTAGGGCCAGTCCAAAAATCGTAACCACCGCT6860.10108973556045121No Hit
TTATTACTGTGCGAGAGCCCACAGCGGTGGTTACGATTTTTGGACTGGCC6830.10064765216878743No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)