FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281386

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281386
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences678605
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATATACACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGG32270.4755343682996736No Hit
GTAATGGATATAACTATTTGGATTGGTACTTGCAGAAGCCAGGGCATTCT20200.29766948372027907No Hit
GTAATAAAGGGCCGTGTCCTCAGATCTCAGGCTGCGCAGTTCCATGTAAA19730.29074351058421316No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT18710.27571267526764465No Hit
ACCTAGGGCCAGTCCAAAAATCGTAACCACCGCTGTGGGCTCTCGCACAG18210.268344618739915No Hit
GTCTGGGACTGAGGTGAAGAAGCCTGGGGCCTCAGTGAGGATTTCCTGCA15300.2254625297485282No Hit
GTGTATATAGTAACTGGTGAAGGTGTATCCAGATGCCTTGCAGGAAATCC12040.1774228011877307No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11550.1702021057905556No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11430.16843377222390052No Hit
ACTATATACACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATG10480.15443446482121412No Hit
GGCCCTAGGTACTACTACTATTACGGTTTGGACGTCTGGGGCCAAGGGAC10410.15340293690733195No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA9920.14618224151015685No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT9740.14352974116017417No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT9510.14014043515741853No Hit
CCTAGGGCCAGTCCAAAAATCGTAACCACCGCTGTGGGCTCTCGCACAGT9470.13955099063520016No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC9170.13513015671856235No Hit
GATTTTACACTGAGAATCAGTAGAGTGGAGGCTGAGGATGTTGGGGTTTA8800.12967779488804237No Hit
GGATATAACTATTTGGATTGGTACTTGCAGAAGCCAGGGCATTCTCCGCA8480.12496223871029538No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8480.12496223871029538No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC8460.1246675164491862No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT8390.12363598853530405No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG7900.11641529313812894No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA7870.11597320974646518No Hit
GTCTACGCACAGAAGTTCCAGGGCAGAGTCACCGTGACCAGGGACACGTC7840.1155311263548014No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7720.11376279278814626No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG7580.11169973696038196No Hit
TCCAACAACCACATCCCTTCTCTACAGAAGCCTCTGAGAGGAAAGTTCTT7530.110962931307609No Hit
GTCCCCACTCTCCCTGTCCGTCACCCCTGGAGAGCCGGCCTCTATTTCCT7370.1086051532187355No Hit
CTTTATTACTGTGCGAGAGCCCACAGCGGTGGTTACGATTTTTGGACTGG7320.10786834756596252No Hit
CCCCTGGAGAGCCGGCCTCTATTTCCTGCAGGTCAAGTCAGAGCCTCCTG7150.10536320834653443No Hit
CCGTAATAGTAGTAGTACCTAGGGCCAGTCCAAAAATCGTAACCACCGCT7130.10506848608542525No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG7040.1037422359104339No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC6950.10241598573544257No Hit
CTCCCAGGTGCACCTGGTGCAGTCTGGGACTGAGGTGAAGAAGCCTGGGG6940.10226862460488798No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)