FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281387

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281387
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1048289
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTAGG22580.2153986162212901No Hit
GCCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTA15820.15091258231270194No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA15770.15043561460627747No Hit
TCCCTATCCTTGGTTCAGGAAACCACGCACAGAAGTTCCAGGGCAGAGTC15030.14337649255119533No Hit
GTGTATTACTGTGCGACCACTGGATATTGTACTACTGCCACCTGCTCTGA14850.14165940880806724No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12580.12000507493639635No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11870.1132321335051689No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC11360.10836706289963931No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG10800.10302502458768527No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10780.10283423750511549No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA10630.10140333438584208No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG10570.1008309731381327No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)