FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281390

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281390
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1011341
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTATGGTGGCTCCTTCAGTGGTTACTACTGGACCTGGATCCGCCAGAC64720.6399424130931111No Hit
ATAATACACAGCCGCGTCCGCGGCGGTCACAGACCTCAGCCTCAGGGAGA47630.47095885561843137No Hit
GTCCTAATGTATGCTGCATCCACTTTACAAAGTGGGGTCCCATCAAGGTT28130.2781455513026763No Hit
GTGTATTATTGTGCGAGAGGATACGGCTATGGAAAGTACCTAATTGATTA27720.2740915279811656No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT25450.25164608178645975No Hit
CATTAGGACCTTAGGGGCTCTCCCAGGTTTCTGCTGATACCAGCCTAAAT24430.24156046279148183No Hit
TTCCATAGCCGTATCCTCTCGCACAATAATACACAGCCGCGTCCGCGGCG21550.2130834209233088No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18820.18608955831910307No Hit
CAATAATACACAGCCGCGTCCGCGGCGGTCACAGACCTCAGCCTCAGGGA18740.18529852937832048No Hit
ACCATAGACAGCGCAGGTGAGGGACAGGGTCTCCGAAGGCTTCAACAGTC18520.18312319979116837No Hit
GTATTATTGTGCGAGAGGATACGGCTATGGAAAGTACCTAATTGATTATT17240.17046673673864698No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14840.14673586851516948No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC14780.14614259680958253No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA14740.14574708233919123No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14620.14456053892801737No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14580.14416502445762605No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA14470.14307735966405No Hit
AATGTATGCTGCATCCACTTTACAAAGTGGGGTCCCATCAAGGTTCAGCG14260.14100090869449572No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14200.1404076369889088No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13900.1374412784609741No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGTTGATGGTGAGAGTGA13790.13635361366739804No Hit
ATAGGAGACAGAGTCACCATCAGTTGCCGGGCAAGTCAGGGCATTAGAAA13600.13447491993303942No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG13510.133585012374659No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT13500.13348613375706117No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA13210.1306186538467243No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA13000.12854220287717003No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12950.1280478097891809No Hit
CTACTGGACCTGGATCCGCCAGACCCCAGGAAAGGGGTTGGAGTGGATTG12560.1241915437028658No Hit
CTCCAAGATTATCATTTTCCTAGGACGTTCGGCCAAGGGACCAAGGTGGA12090.11954424867576811No Hit
ACTTATTACTGTCTCCAAGATTATCATTTTCCTAGGACGTTCGGCCAAGG12070.11934649144057247No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC12020.11885209835258334No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12000.11865434111738771No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11950.1181599480293986No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC11920.11786331217660512No Hit
ATCTTGGAGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGTTGA11830.11697340461822472No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAG11730.11598461844224649No Hit
ACTACAACCCGTCCCTCAAGAGTCGAGTCGCCATTTCAGTAGACACGTCC11700.11568798258945301No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC11640.11509471088386608No Hit
ATTAGGTACTTTCCATAGCCGTATCCTCTCGCACAATAATACACAGCCGC11620.11489695364867043No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11250.11123844479755096No Hit
GCTATGGAAAGTACCTAATTGATTATTGGGGCCAGGGAACCCTGGTCACC11220.1109418089447575No Hit
GCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGG11100.10975526553358363No Hit
GCCGTATCCTCTCGCACAATAATACACAGCCGCGTCCGCGGCGGTCACAG11000.10876647935760539No Hit
ATACACAGCCGCGTCCGCGGCGGTCACAGACCTCAGCCTCAGGGAGAATT10800.10678890700564893No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC10780.10659114977045328No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG10720.10599787806486637No Hit
CCCTCATGCTGCCACCCCCGACTGTCACTGCACCGACCGGCCCTCGAGGA10660.10540460635927941No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT10600.10481133465369247No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG10570.104514698800899No Hit
GTCCCTCACCTGCGCTGTCTATGGTGGCTCCTTCAGTGGTTACTACTGGA10560.10441582018330117No Hit
TCCTTGAACAGCGCTCTTCCCACTTGAGGGCGTCCAGGTGAAGGTGACAC10490.10372366986011643No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC10380.10263600506654037No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT10310.1019438547433556No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG10180.10065843271458391No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG15200.050.701863
TCAACGC15700.050.4584274
CAACGCA16750.047.295365
TATCAAC16550.046.5706632
AACGCAG17350.045.6462366
ACGCAGA18750.042.2379847
CGCAGAG19150.041.355738
CTAGTAC750.00153208238.413373
CTATGGT27550.035.2935873
TCTATGG27450.035.1632582
GTCTATG27900.034.861191
GTACATG27500.034.58231
GCAGAGT23150.034.2066469
TACATGG27600.033.9282072
GAGTTTA4700.032.190971
GTATCAA29200.031.3352761
ATCGTGC1153.2940082E-431.3028558
GGTGGCT31650.030.936917
TATGGTG32900.030.427034
CATGGGG17600.030.2802684
Produced by FastQC (version 0.11.5)