FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281391

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281391
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1088304
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTCCATTTGCAGATACA60580.5566459371646157No Hit
GTGTATTACTGTGCGAGAGCTGGAGGGGGTGACTACATAAACTACTGGGG51850.47642938002616914No Hit
GTTATATATATTACGCAGACTCAGTGAAGGGCCGATTCGCCATCTCCAGA37680.3462267895735015No Hit
ATATATAACTACTACCACTACTAATGGACGAGACCCAGTCCAGTCCCTTC37640.34585924521089695No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT35500.32619562181155265No Hit
ATATTACGCAGACTCAGTGAAGGGCCGATTCGCCATCTCCAGAGACAACG35060.32215263382290243No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA33140.30451050441788324No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT31480.28925741336979377No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC25710.23623913906408506No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT25370.23311501198194623No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG25340.2328393537099928No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT24220.22254811155706494No Hit
GCCCAGGGCCGCTGTACTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG23660.217402490480601No Hit
GCGTAATATATATAACTACTACCACTACTAATGGACGAGACCCAGTCCAG23480.21574854084888045No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGG20370.18717196665637542No Hit
CTCCAGAGACAACGCCAAGAACTCACTGTATCTGCAAATGGACAGCCTGA20320.18671253620311973No Hit
ACATAAACTACTGGGGCCAGGGAATCCTGGTCACCGTCTCCTCAGCCTCC19660.18064805422014438No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACA17980.16521119099075257No Hit
CACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA17240.1584116202825681No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTAAATCTACC17110.15721710110410328No Hit
GTATTACTGTGCGAGAGCTGGAGGGGGTGACTACATAAACTACTGGGGCC16860.15491994883782473No Hit
ATACACAGCCGTGTCCTCGGCTCTCAGGCTGTCCATTTGCAGATACAGTG16850.1548280627471736No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTAAATCTACCATGAATTGGG16730.1537254296593599No Hit
GTACATGGGAGCTCTGAGAGAGGAGCCTTAGCCCTGGATTCCAAGGCCTA16660.1530822270248019No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG16420.15087696084917449No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAAC16050.14747717549508224No Hit
GTGTAGGTCTGGGTGCCGAAGTTGCTGGAGGGCACGGTCACCACGCTGCT15890.146006998044664No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGA15710.14435304841294344No Hit
GCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGGTTTTC15520.14260721269057175No Hit
TTTAGAAGGTGTCCATTGTGAGGTGCAGCTGGTTGAGTCTGGGGGAGGCC15490.1423315544186183No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA15370.14122892133080464No Hit
GCTGTGTATTACTGTGCGAGAGCTGGAGGGGGTGACTACATAAACTACTG14770.13571575589173612No Hit
CAGTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTCCATTTGCAGATA14500.1332348314441553No Hit
TCCTGGAGCAGGGCGCCAGGGGGAAGACCGATGGGCCCTTGGTGGAGGCT14200.13047824872462105No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG14150.13001881827136536No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCT14070.12928372954615622No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTG13940.1280892103676914No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA13920.12790543818638908No Hit
GGTTGATACTTACCCCCTCAGTTTCGGCGGAGGGACCAAGGTGGACATCA13920.12790543818638908No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTACTCTCGGAGGTGCTCCTGG13870.1274460077331334No Hit
AGTTTATGTAGTCACCCCCTCCAGCTCTCGCACAGTAATACACAGCCGTG13850.1272622355518311No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13830.1270784633705288No Hit
GTCCACCACCACGCACGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT13820.1269865772798777No Hit
CCGCTGGTCAGAGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA13730.12615960246401742No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT13670.12560828592011056No Hit
AGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTC13450.12358679192578544No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT13090.12027889266234436No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAAC13040.11981946220908862No Hit
CCATGGAACTGGGGCTCCGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGT13010.11954380393713522No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAA12920.11871682912127494No Hit
GTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGA12840.11798174039606579No Hit
GCTCCAGGAGCACCTCCGAGAGTACAGCGGCCCTGGGCTGCCTGGTCAAG12770.11733853776150782No Hit
ATTTTAGAAGGTGTCCATTGTGAGGTGCAGCTGGTTGAGTCTGGGGGAGG12650.11623590467369412No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAG12580.11559270203913613No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC12470.11458195504197356No Hit
GCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGTTCCACGACAC12370.11366309413546215No Hit
TTCCAAGGCCTATCCACTTGGTGATCAGCACTGAGCACCGAGGATTCACC12160.11173348623178818No Hit
CTACAAGACCACGCCTCCCATGCTGGACTCCGACGGCTCCTTCTTCCTCT12140.11154971405048589No Hit
CTGTTGAACTGCTCCTCCCGTGGCTTTGTCTTGGCATTATGCACCTCCAC12030.11053896705332333No Hit
GTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAAC11970.10998765050941649No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGC11930.10962010614681192No Hit
GTTCAACAGCACGTTCCGTGTGGTCAGCGTCCTCACCGTCGTGCACCAGG11890.10925256178420735No Hit
GGACTGGACTGGGTCTCGTCCATTAGTAGTGGTAGTAGTTATATATATTA11790.10833370087769593No Hit
GCCACAGGTGGTGCTGGGCACGGTGGGCACTCGACACAACATTTGCGCTC11710.1075986121524868No Hit
GTCTGGGGGAGGCCTGGTCAAGCCTGGGGGGTCCCTGAGACTCTCCTGTG11550.10612843470206854No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)