FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1088304
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTCCATTTGCAGATACA48480.44546376747673444No Hit
GTGTATTACTGTGCGAGAGCTGGAGGGGGTGACTACATAAACTACTGGGG45250.4157845601964157No Hit
GTTATATATATTACGCAGACTCAGTGAAGGGCCGATTCGCCATCTCCAGA35200.3234390390920184No Hit
ATATATAACTACTACCACTACTAATGGACGAGACCCAGTCCAGTCCCTTC31770.29192210999867685No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT31600.29036004645760743No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA28980.2662858907070083No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT28690.26362119407812523No Hit
ATATTACGCAGACTCAGTGAAGGGCCGATTCGCCATCTCCAGAGACAACG28520.2620591305370558No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC21720.19957658889427954No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT21060.19351210691130422No Hit
GCCCAGGGCCGCTGTACTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG21020.19314456254869963No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG20970.19268513209544394No Hit
GCGTAATATATATAACTACTACCACTACTAATGGACGAGACCCAGTCCAG20700.1902042076478631No Hit
GTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGA18190.16714079889442657No Hit
CTCCAGAGACAACGCCAAGAACTCACTGTATCTGCAAATGGACAGCCTGA18120.16649759625986857No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGG16120.14812037812964024No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT15730.14453682059424575No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT15010.13792102206736354No Hit
ACATAAACTACTGGGGCCAGGGAATCCTGGTCACCGTCTCCTCAGCCTCC14760.13562386980108498No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTAAATCTACC14280.1312133374498302No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA14200.13047824872462105No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTAAATCTACCATGAATTGGG14140.12992693218071422No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGA13980.12845675473029594No Hit
GTACATGGGAGCTCTGAGAGAGGAGCCTTAGCCCTGGATTCCAAGGCCTA13850.1272622355518311No Hit
GTATTACTGTGCGAGAGCTGGAGGGGGTGACTACATAAACTACTGGGGCC13760.12643526073597083No Hit
GTGTAGGTCTGGGTGCCGAAGTTGCTGGAGGGCACGGTCACCACGCTGCT13760.12643526073597083No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACA13150.1208302092062512No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC13020.11963569002778635No Hit
CACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA12950.11899248739322837No Hit
GCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGGTTTTC12930.11880871521192607No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACGACGGTGAGGACGC12930.11880871521192607No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG12700.11669533512694982No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA12640.11614401858304298No Hit
GGTTGATACTTACCCCCTCAGTTTCGGCGGAGGGACCAAGGTGGACATCA12200.11210103059439275No Hit
ATACACAGCCGTGTCCTCGGCTCTCAGGCTGTCCATTTGCAGATACAGTG12030.11053896705332333No Hit
GCTGTGTATTACTGTGCGAGAGCTGGAGGGGGTGACTACATAAACTACTG11930.10962010614681192No Hit
GTCCACCACCACGCACGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT11800.10842558696834707No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTACTCTCGGAGGTGCTCCTGG11750.10796615651509137No Hit
CAGTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTCCATTTGCAGATA11640.10695540951792881No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT11600.10658786515532424No Hit
AGTTTATGTAGTCACCCCCTCCAGCTCTCGCACAGTAATACACAGCCGTG11600.10658786515532424No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCT11530.10594466252076624No Hit
TTTAGAAGGTGTCCATTGTGAGGTGCAGCTGGTTGAGTCTGGGGGAGGCC11400.10475014334230143No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG11370.10447448507034798No Hit
GCTCCAGGAGCACCTCCGAGAGTACAGCGGCCCTGGGCTGCCTGGTCAAG11340.10419882679839455No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAG11330.10410694070774341No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAAC11220.10309619371058087No Hit
AGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTC11050.10153413016951146No Hit
CCGCTGGTCAGAGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA10960.10070715535365118No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)