FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281393

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281393
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences749757
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT18500.24674661256913907No Hit
ATCTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGT17730.23647661842436948No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGAGCTCAGCTTCAGGGAGA15950.2127355929987983No Hit
GTATTACTGTCAGCAGTATGGTAGCTCACCGTACACTTTTGGCCAGGGGA14830.19779741969731526No Hit
GTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCC13760.1835261291325056No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12090.16125224572761573No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10800.14404667112144334No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10710.1428462821954313No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT10290.13724446720737518No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGT10100.13471031280801646No Hit
GTAATAGATATACCCAATCCACTCCAGTCCCTTCCCTGGGGGCTGCCGGA9890.1319094053139884No Hit
GGGTATATCTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAG9750.13004213365130302No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT9610.12817486198861766No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9430.1257740841365936No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA9230.1231065531899002No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC8640.11523733689715468No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC8540.11390357142380797No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8370.1116361701191186No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC8350.11136941702444925No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA8220.10963552190909855No Hit
GTGTATTACTGTGCGAGGACAGACTCAGATTACTATGATAGTAGTGGTTC8200.10936876881442921No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT8010.10683461441507047No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG7930.10576760203639313No Hit
GTATGGTAGCTCACCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCA7660.10216643525835704No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG7560.10083266978501033No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC7520.10029916359567168No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG25300.051.519023
TCAACGC25350.051.410554
CAACGCA26350.049.459485
TATCAAC27100.048.097092
CCGATCG450.009908820547.9957169
AACGCAG27400.047.5641376
CGCAGAG28900.045.0893978
GCAGAGT30900.042.1709869
ACGCAGA33250.039.190487
GTATCAA38550.037.0043871
CAGCGCT3100.032.513229
TACACCG1753.980449E-628.8012685
TATACTG4150.027.7602565
ATACACG11050.026.0644974
CTATACT3751.2732926E-1124.9610984
GAATACG2601.2242162E-724.9208499
AGCGCTG3801.4551915E-1124.6326645
GTCCTAT2051.353972E-524.6012171
ATAGGTG2351.411754E-624.5117175
TCTACAC6200.024.3914223
Produced by FastQC (version 0.11.5)