FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281394

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281394
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences749757
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT19730.2631519278913034No Hit
ATCTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGT17610.23487609985635344No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGAGCTCAGCTTCAGGGAGA15300.20406611742204475No Hit
GTATTACTGTCAGCAGTATGGTAGCTCACCGTACACTTTTGGCCAGGGGA15130.20179871611735534No Hit
GTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCC13040.17392301772440938No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11570.1543166652662129No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT11550.15404991217154357No Hit
GTAATAGATATACCCAATCCACTCCAGTCCCTTCCCTGGGGGCTGCCGGA11420.15231601705619288No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11210.14951510956216482No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGT10070.13431018316601245No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9270.12364005937923886No Hit
GGGTATATCTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAG9260.1235066828319042No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9250.12337330628456954No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT9150.12203954081122284No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC9060.1208391518852108No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA8820.11763811474917875No Hit
GTGTATTACTGTGCGAGGACAGACTCAGATTACTATGATAGTAGTGGTTC8780.11710460855984006No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8350.11136941702444925No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA8230.10976889845643323No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC8160.10883526262509052No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA7950.10603435513106246No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT7780.10376695382637309No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC7720.10296669454236507No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG7590.10123279942701435No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)