FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281395

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281395
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences872070
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAATAGCCGTGTCTGCGGCGGTCACGGAGCTCAGCTTCAGGGAGA51880.5949063721948926No Hit
TAATAATAGAGGCCGCTCACACAGTAATAAATAGCCGTGTCTGCGGCGGT44060.5052346715286616No Hit
ATTTATTACTGTGTGAGCGGCCTCTATTATTATGATAGTAGTGATTATTA40970.4698017360991663No Hit
CTATTATTATGATAGTAGTGATTATTACTTTCCCAACTTTGACTACTGGG38660.4433130367975048No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT27100.31075487059525037No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA25800.29584781038219404No Hit
ATTTACTACTGGGCCTGGATCCGTCAGCCCCCAGGGAAGGGACTGGAGTG23230.2663776990379213No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT23150.26546034148634856No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC22480.25777747199192724No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC21420.24562248443358906No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA20430.23427018473287697No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG19820.22727533340213515No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC18680.21420298829222423No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT18250.209272191452521No Hit
GATTATTACTTTCCCAACTTTGACTACTGGGGCCGGGGAACCCTGGTCGC18030.2067494581856961No Hit
GTAATAATCACTACTATCATAATAATAGAGGCCGCTCACACAGTAATAAA18000.20640544910385636No Hit
GTATGAAAGTGGGACGACCTTCTACAATCCGTCTCTCAAGAGTCGAGTCT17310.19849324022154186No Hit
CAGTAATAAATAGCCGTGTCTGCGGCGGTCACGGAGCTCAGCTTCAGGGA17030.19528248879103743No Hit
TTATTACTGTGTGAGCGGCCTCTATTATTATGATAGTAGTGATTATTACT16420.18828763746029561No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG16150.18519155572373777No Hit
TCCGTAGACACGTCCAAGGCCCAGTTCTCCCTGAAGCTGAGCTCCGTGAC16120.184847546641898No Hit
ACCTTCTACAATCCGTCTCTCAAGAGTCGAGTCTCCATATCCGTAGACAC15220.1745272741867052No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT14310.1640923320375658No Hit
GTAGAAGGTCGTCCCACTTTCATACATACTCGCAATCCACTCCAGTCCCT14110.16179893815863405No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT14010.16065224121916818No Hit
CTACTATCATAATAATAGAGGCCGCTCACACAGTAATAAATAGCCGTGTC13470.1544600777460525No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA13440.15411606866421274No Hit
GCTTGATACCACTGCTTGATACCACTGCTTGATACCACTGCTTGATACCA13280.15228135356106734No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG13170.1510199869276549No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG13090.1501026293760822No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT12900.14792390519109702No Hit
GTCTCTAGTGGCAGTAGTATTTACTACTGGGCCTGGATCCGTCAGCCCCC12750.14620385978189823No Hit
TGATAGTAGTGATTATTACTTTCCCAACTTTGACTACTGGGGCCGGGGAA12720.14585985070005847No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA12680.14540117192427215No Hit
GTCTACGGATATGGAGACTCGACTCTTGAGAGACGGATTGTAGAAGGTCG12600.14448381437269944No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT12590.14436914467875286No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC12420.1424197598816609No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC12000.13760363273590423No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG11930.13680094487827812No Hit
GTATCAAGCAGTGGTATCAAGCAGTGGTATCAAGCAGTGGTATCAAGCAG11930.13680094487827812No Hit
AATAGAGGCCGCTCACACAGTAATAAATAGCCGTGTCTGCGGCGGTCACG11910.13657160549038494No Hit
GATATGGAGACTCGACTCTTGAGAGACGGATTGTAGAAGGTCGTCCCACT11750.13473689038723957No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC11670.13381953283566686No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT11660.13370486314172028No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC11610.13313151467198733No Hit
GTATTTACTACTGGGCCTGGATCCGTCAGCCCCCAGGGAAGGGACTGGAG11600.13301684497804075No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA11470.13152613895673512No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG11290.12946208446569657No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11280.12934741477174996No Hit
TCTACAATCCGTCTCTCAAGAGTCGAGTCTCCATATCCGTAGACACGTCC11080.12705402089281823No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG10960.1256779845654592No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC10930.12533397548361944No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG10830.12418727854415355No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAA10730.1230405816046877No Hit
GTCATGGATCTCATGTGCAAGAAAATGAAGCACCTGTGGTTCTTCCTCCT10530.12074718772575596No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT10370.11891247262261057No Hit
CTCCATATCCGTAGACACGTCCAAGGCCCAGTTCTCCCTGAAGCTGAGCT10260.11765110598919812No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC10090.11570172119210614No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAG9970.11432568486474709No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT9920.11375233639501416No Hit
GCCTCTATTATTATGATAGTAGTGATTATTACTTTCCCAACTTTGACTAC9830.11272030914949488No Hit
CACCTGCAGTGTCTCTAGTGGCAGTAGTATTTACTACTGGGCCTGGATCC9630.11042691527056316No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC9440.10824819108557798No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC9440.10824819108557798No Hit
CTCCTGGGGGGACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACAC9420.10801885169768483No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT9410.10790418200373823No Hit
ATTATTATGATAGTAGTGATTATTACTTTCCCAACTTTGACTACTGGGGC9260.10618413659453942No Hit
CACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA9260.10618413659453942No Hit
CATAATAATAGAGGCCGCTCACACAGTAATAAATAGCCGTGTCTGCGGCG9180.10526677904296675No Hit
CTTTCATACATACTCGCAATCCACTCCAGTCCCTTCCCTGGGGGCTGACG9080.10412008210350086No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA9040.10366140332771451No Hit
ATATAATGATTACTCGTACACTTTTGGCCAGGGGACCAAACTGGAGATCA9000.10320272455192818No Hit
GTCTCAGGTGCACGTGCAGGAGTCGGGCCCTGGACTGGTGAGGCCTTCGG8980.102973385164035No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC8940.10251470638824864No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGC8930.10240003669430206No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT8840.10136800944878278No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG15450.064.31463
TCAACGC15800.062.8862954
CAACGCA16450.060.397975
TATCAAC16600.060.2928282
AACGCAG17250.057.59696
CGCAGAG19050.052.1546758
GCAGAGT21650.046.2238439
CAGCGCT6800.044.4681979
GGCAGCG7000.042.1691637
GCAGCGC7200.040.9977958
CAGGGCA8350.037.0779764
GTATTAG2600.036.0187151
GTATCAA32000.034.893131
ACGCAGA29900.033.7105647
TATAGAC1751.07160304E-732.916293
GTACATG20150.030.7455061
TAATAGA23950.030.3633654
TACATGG19650.030.047632
GTACTAG1204.2126572E-430.0155961
ATAGAGG24200.029.7504656
Produced by FastQC (version 0.11.5)