FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281396

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281396
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences872070
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAATAGCCGTGTCTGCGGCGGTCACGGAGCTCAGCTTCAGGGAGA50100.5744951666724002No Hit
TAATAATAGAGGCCGCTCACACAGTAATAAATAGCCGTGTCTGCGGCGGT44100.5056933503044481No Hit
ATTTATTACTGTGTGAGCGGCCTCTATTATTATGATAGTAGTGATTATTA43120.4944557202976825No Hit
CTATTATTATGATAGTAGTGATTATTACTTTCCCAACTTTGACTACTGGG38990.44709713669774215No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT31100.3566227481738851No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA25110.2879356014998796No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC22860.26213492036189756No Hit
ATTTACTACTGGGCCTGGATCCGTCAGCCCCCAGGGAAGGGACTGGAGTG22820.2616762415861112No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT21010.24092102698177897No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA19960.22888070911738734No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC19930.2285367000355476No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC19100.21901911543798092No Hit
GTAATAATCACTACTATCATAATAATAGAGGCCGCTCACACAGTAATAAA18230.20904285206462783No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG18130.20789615512516196No Hit
GTATGAAAGTGGGACGACCTTCTACAATCCGTCTCTCAAGAGTCGAGTCT18120.20778148543121538No Hit
GATTATTACTTTCCCAACTTTGACTACTGGGGCCGGGGAACCCTGGTCGC17170.19688786450628962No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT16690.19138371919685346No Hit
TTATTACTGTGTGAGCGGCCTCTATTATTATGATAGTAGTGATTATTACT16350.1874849496026695No Hit
CAGTAATAAATAGCCGTGTCTGCGGCGGTCACGGAGCTCAGCTTCAGGGA16260.18645292235715022No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA16010.18358618000848556No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG15440.1770500074535301No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT15340.17590331051406424No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT15330.17578864082011766No Hit
ACCTTCTACAATCCGTCTCTCAAGAGTCGAGTCTCCATATCCGTAGACAC15220.1745272741867052No Hit
TCCGTAGACACGTCCAAGGCCCAGTTCTCCCTGAAGCTGAGCTCCGTGAC14980.1717752015319871No Hit
GCTTGATACCACTGCTTGATACCACTGCTTGATACCACTGCTTGATACCA14030.16088158060706137No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG13650.15652413223709105No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT13450.1542307383581593No Hit
CTACTATCATAATAATAGAGGCCGCTCACACAGTAATAAATAGCCGTGTC13440.15411606866421274No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT13340.15296937172474687No Hit
GTAGAAGGTCGTCCCACTTTCATACATACTCGCAATCCACTCCAGTCCCT13320.1527400323368537No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC13280.15228135356106734No Hit
GTCTCTAGTGGCAGTAGTATTTACTACTGGGCCTGGATCCGTCAGCCCCC12940.1483825839668834No Hit
GTCTACGGATATGGAGACTCGACTCTTGAGAGACGGATTGTAGAAGGTCG12770.14643319916979142No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT12590.14436914467875286No Hit
TGATAGTAGTGATTATTACTTTCCCAACTTTGACTACTGGGGCCGGGGAA12370.14184641141192794No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG12250.1404703750845689No Hit
GTATTTACTACTGGGCCTGGATCCGTCAGCCCCCAGGGAAGGGACTGGAG12110.13886499936931668No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG12100.1387503296753701No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC11840.13576891763275883No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG11760.13485156008118615No Hit
GATATGGAGACTCGACTCTTGAGAGACGGATTGTAGAAGGTCGTCCCACT11590.13290217528409415No Hit
GTATCAAGCAGTGGTATCAAGCAGTGGTATCAAGCAGTGGTATCAAGCAG11570.132672835896201No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT10980.12590732395335238No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA10970.12579265425940578No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA10870.12464595731993991No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC10700.12269657252284792No Hit
CTCCATATCCGTAGACACGTCCAAGGCCCAGTTCTCCCTGAAGCTGAGCT10650.122123224053115No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC10590.12143520588943549No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC10510.12051784833786278No Hit
TCTACAATCCGTCTCTCAAGAGTCGAGTCTCCATATCCGTAGACACGTCC10480.12017383925602304No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG10270.11776577568314471No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT10260.11765110598919812No Hit
GTCATGGATCTCATGTGCAAGAAAATGAAGCACCTGTGGTTCTTCCTCCT10220.11719242721341178No Hit
AATAGAGGCCGCTCACACAGTAATAAATAGCCGTGTCTGCGGCGGTCACG10110.1159310605799993No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC10060.11535771211026638No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG9940.11398167578290734No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9860.11306431823133464No Hit
ATTATTATGATAGTAGTGATTATTACTTTCCCAACTTTGACTACTGGGGC9720.11145894251608243No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAG9710.11134427282213583No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT9680.11100026374029609No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC9540.10939488802504387No Hit
GCCTCTATTATTATGATAGTAGTGATTATTACTTTCCCAACTTTGACTAC9530.10928021833109727No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT9510.1090508789432041No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC9440.10824819108557798No Hit
CCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAG9230.10584012751269968No Hit
CATAATAATAGAGGCCGCTCACACAGTAATAAATAGCCGTGTCTGCGGCG9230.10584012751269968No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCA9100.10434942149139405No Hit
CACCTGCAGTGTCTCTAGTGGCAGTAGTATTTACTACTGGGCCTGGATCC9000.10320272455192818No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAA8960.10274404577614182No Hit
GTCTCAGGTGCACGTGCAGGAGTCGGGCCCTGGACTGGTGAGGCCTTCGG8770.10056532159115666No Hit
GTCTTGGCATTATGCACCTCCACGCCGTCCACGTACCAGTTGAACTTGAC8750.1003359822032635No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)