FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281397

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281397
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2658233
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT33900.12752832426653346No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32910.12380404577025415No Hit
GTATGTGTTGGTGCCACTGTCACTAATGCCTGAGACCCACTCCAGCCCCT29060.10932074050694576No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC29040.109245502557526No Hit
AACACATACTATGCAGCGTCTGTGCAGGGGCGCTTCTCCATGTCCAGAGA28910.1087564558862974No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT28480.10713883997377205No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA27160.10217313531206633No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC27110.10198504043851686No Hit
ATACTATGCAGCGTCTGTGCAGGGGCGCTTCTCCATGTCCAGAGACAATT26930.10130789889373881No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA52200.076.453351
ATCAACG60250.064.415873
TCAACGC63100.061.620544
TATCAAC65950.059.2851872
CAACGCA69900.055.8309565
AACGCAG71650.054.46636
CGCAGAG80200.048.478358
GCAGAGT90500.042.9609269
ACGCAGA95900.040.5418557
GGTATCA38350.037.5683331
GTACATG77350.032.3166541
TACATGG76650.032.0334242
ACATGGG77350.031.2780823
GAATACG5150.029.3589529
CAGAGTA90650.026.8458619
GTACACG16400.025.903665
CATGGGA38550.025.215674
ACCGTTC3451.1823431E-1025.0432458
CATGGGG49400.024.7789614
TACACGG17850.024.2023666
Produced by FastQC (version 0.11.5)