FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281398

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281398
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2658233
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31170.11725834417073296No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT29910.11251835335728659No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT28610.10762788664500066No Hit
GTATGTGTTGGTGCCACTGTCACTAATGCCTGAGACCCACTCCAGCCCCT26700.1004426624754113No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA49250.074.414851
ATCAACG54200.066.0269853
TCAACGC56250.063.876694
TATCAAC60450.059.5577242
CAACGCA68700.052.509425
AACGCAG71050.050.4695786
CGCAGAG73550.048.2645878
GCAGAGT83150.042.6008459
ACGCAGA89350.039.891017
GGTATCA38800.033.403261
GTACATG72600.032.6292651
TACATGG72250.032.290262
ACATGGG72650.031.7160243
CATGGGA37050.026.4310914
CAGAGTA81350.025.134849
TCTACAC18400.024.2626513
TTAACGC1550.001882788523.2275123
CATGGGG44650.022.7385124
TAACGCA3004.8714537E-721.6015834
GTATATA13050.020.9662671
Produced by FastQC (version 0.11.5)