FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281399

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281399
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2997357
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT114170.38090224154146474No Hit
ATATTACCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA113660.3792007425208275No Hit
GAGTTATATTACCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCA86320.2879870499243166No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC83620.278979113932708No Hit
GTATTACTGTACGATCCCGACCACAATTAATCAGATCGCTGACTACTGGG73840.246350368007548No Hit
GTGCATCCACTTTGCAAAGTGGGGTCCCATCCAGGTTCACTGGCAGTGGT72830.24298073269216847No Hit
ATATAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACT69250.23103687682181334No Hit
GGGTAATATAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGC68110.22723352606980082No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT59700.19917547359223475No Hit
GTTGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAACAT58650.19567238737327586No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG54190.18079261162417423No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC52760.17602174182121116No Hit
ATACAGGGCCGTGTCGTCAGAGGTGAGGCTGCTCAGTTCCATGTAGGCTG52590.17545457548099877No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC51680.1724185674245677No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG51460.17168458745488108No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG49810.16617973768223138No Hit
GCCCTGTATTACTGTACGATCCCGACCACAATTAATCAGATCGCTGACTA48060.16034126065063323No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT47040.15693826260935884No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG46760.15600410628430314No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT46560.15533685176640621No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG45970.15336845093861026No Hit
GTAATACAGGGCCGTGTCGTCAGAGGTGAGGCTGCTCAGTTCCATGTAGG45900.15313491185734635No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT45890.1531015491314515No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA45470.1517003146438679No Hit
CAGTAATACAGGGCCGTGTCGTCAGAGGTGAGGCTGCTCAGTTCCATGTA45410.15150013828849884No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC44210.14749661118111723No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC43570.14536139672384704No Hit
GTGGATGCACCGTGGATCAGGAGCTTAGGGGCTTTGCCGGGTTTTTGCTG40460.13498558897054974No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG40420.13485213806697033No Hit
ACTTACTACTGTCAACAGAGTTATATTACCCCTCGGACGTTCGGCCAAGG40340.13458523625981156No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA39500.13178276728464444No Hit
TTCCAGGACAGAGTCACGATTACCGCGGACGAGTCTACGACCACAGCCTA39430.1315492282033805No Hit
GTCCTGGAACTTCTGTGCGTAGTGTGCTCTACCAAACACAGGGATGATCC39050.13028144461937632No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG38160.12731216201473497No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAAC37860.12631128023788957No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC37590.12541048663872872No Hit
GTCTACGACCACAGCCTACATGGAACTGAGCAGCCTCACCTCTGACGACA37570.12534376118693902No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT37300.12444296758777817No Hit
ACATGGAACTGAGCAGCCTCACCTCTGACGACACGGCCCTGTATTACTGT36110.12047280320629142No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGCGTGA35930.11987227414018417No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35860.11963873505892024No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC35680.11903820599281299No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA35610.11880466691154908No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA35260.11763697150522943No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA35040.11690299153554282No Hit
CATAACAACCACATTCCTCCTCTAAAGAAGCCCCTGGGAGCACAGCTCAT34880.11636918792122526No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC34420.11483450253006232No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA34280.11436742436753446No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT34280.11436742436753446No Hit
ATCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA33990.11339990531658391No Hit
CCTCTAAAGAAGCCCCTGGGAGCACAGCTCATCACCATGGCCTGGACCTG33980.11336654259068905No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33650.11226557263615913No Hit
CCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAG33240.11089770087447041No Hit
GTTATATTACCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA32520.10849558461004144No Hit
CTGTATTACTGTACGATCCCGACCACAATTAATCAGATCGCTGACTACTG32320.10782833009214451No Hit
GTCTCCATCTTCCCTGTCTGCATCTGTTGGAGACAGAGTCACCATCACTT32310.10779496736624966No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG32210.10746134010730118No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT32060.1069608992188785No Hit
GCTCATCACCATGGCCTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG31840.10622691924919186No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG31170.10399161661423714No Hit
GTCGTAGACTCGTCCGCGGTAATCGTGACTCTGTCCTGGAACTTCTGTGC31090.10372471480707837No Hit
TTACTGTACGATCCCGACCACAATTAATCAGATCGCTGACTACTGGGGCC30920.10315754846686596No Hit
CCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTG30250.10092224583191124No Hit
GTTCTGGGACAGATTTCACGCTCACCATCAGCAGTCTGCAACCTGAAGAT30180.10068870675064732No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG29990.10005481495864524No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA52550.084.859071
ATCAACG64800.067.672543
TCAACGC66150.066.2914664
TATCAAC70100.062.7594152
CAACGCA74650.058.7432065
AACGCAG78850.055.6104976
CGCAGAG84750.051.6541338
GCAGAGT94200.046.5487029
ACGCAGA105050.041.7409637
CAGCGCT17150.037.365189
GTACATG86350.032.5373841
ACATGGG87450.031.5361843
CATGGGG46700.029.604284
TATACGG1255.3666555E-428.8024985
CAGGGCA23550.028.4355894
GCAGCGC27550.025.6121278
GTATGTA36500.025.4611021
GTACACG29700.025.4567555
AGCGCTG18300.024.395565
TACACGG31050.024.3483146
Produced by FastQC (version 0.11.5)