FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281402

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281402
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2006879
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCCTCGGCTCTCAGGCCATTCATTTGCAGAAACA47860.2384797489036459No Hit
GTCTATTACTGTGTGAGAACCCGAGATTGTTCTGGTGATAGTTGCTATCC47630.23733369077059452No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGACTCTG30740.1531731609130396No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC30070.14983464374284647No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29430.14664561241609483No Hit
GTAATAGGCGGCCTCATCCCCGGCCTCGACCCTGCTGATGGTCAGGGTGG27570.1373774901227229No Hit
CTACTATCCCACACGTGACAGTAATAGGCGGCCTCATCCCCGGCCTCGAC24390.1215319907179257No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT24190.1205354184283158No Hit
ATACTACGCAGACTCAGTGAAGGGCCGATTCACCATCTCCAGAGACAACG22820.11370889824448807No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA22250.11086866721909991No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT21940.10932398017020459No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT21670.10797860757923124No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT21670.10797860757923124No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA20790.10359368950494774No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA20480.10204900245605242No Hit
GACGTAGGACGGTCAGCTCGGTCCCTCCGCCGAACACCCAATGATTACCA20160.10045448679267659No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA42900.073.6772461
ATCAACG51100.060.871033
TCAACGC51650.060.362254
TATCAAC53400.058.5189172
CAACGCA58000.053.629485
AACGCAG59500.052.520816
CGCAGAG66500.046.775758
GCAGAGT73100.042.3502129
TATTGAG24450.032.0992935
ACGCAGA96500.032.0848357
GTACATG66500.030.7484261
TACATGG66250.030.4312632
ACATGGG66500.030.1003133
AATAGAC27500.028.539193
CATGGGA31200.028.154844
CAGCCGT26450.027.7638079
TAGACAG31100.027.5508125
TTATTGA28700.027.3459134
GTGCTAG6950.026.934861
TTGAGGG15600.026.7708447
Produced by FastQC (version 0.11.5)