FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281403

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281403
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences867422
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATAACCACTCCAAAACTATCACCTGTCGCACAGTAATACACGGCC25010.2883256361955311No Hit
GTGCAGGGTCGTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT23110.26642164943937324No Hit
AGGTATACTGTGCAGTGGGTGCGACAGGCTCGTGGACAACGCCTTGAGTG21830.2516652794141721No Hit
GTAATACACGGCCGTGTCCTCGGATCTCAGGCTGCTCAACTCCATGTAGG21640.2494748807385563No Hit
GTTATAAGGGAGACCTACTACTATGCTTTGGACGTCTGGGGCCAAGGGAC20470.23598663626239594No Hit
CTAGGTATACTGTGCAGTGGGTGCGACAGGCTCGTGGACAACGCCTTGAG19810.2283778829681516No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC19580.22572634772924827No Hit
GTAGTAGGTCTCCCTTATAACCACTCCAAAACTATCACCTGTCGCACAGT19000.21903986756157903No Hit
GGTATACTGTGCAGTGGGTGCGACAGGCTCGTGGACAACGCCTTGAGTGG17630.20324594026898093No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17010.19609832353802417No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15370.17719172444323525No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAATACTAAGG14440.16647029934680008No Hit
GTTCCTCACTATGGACTGGATTTGGAGGATCCTCTTCTTGGTGGGAGCAG13420.15471131698296794No Hit
GTCTGGGCCTGAGGTGAAGAAGCCTGGGACCTCAGTGAAGGTCTCCTGCA12980.14963881478680505No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12890.1486012575194081No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT12440.1434134711824233No Hit
TACTAAGGCTGTCATCCCATGCTGCACAGAAATAATCAGCCTCATCTTCG12420.14318290290077954No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC12210.14076193594352No Hit
CCCAAATACTAAGGCTGTCATCCCATGCTGCACAGAAATAATCAGCCTCA11830.1363811385922884No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA11810.13615057031064465No Hit
GTGTATTACTGTGCGACAGGTGATAGTTTTGGAGTGGTTATAAGGGAGAC11740.13534358132489147No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC11510.13269204608598814No Hit
ACCTACTACTATGCTTTGGACGTCTGGGGCCAAGGGACCACGGTCACCGT11250.12969465842461916No Hit
GCCTACATGGAGTTGAGCAGCCTGAGATCCGAGGACACGGCCGTGTATTA11190.12900295357968786No Hit
CCTCACTCACTGTGCAGGGTCGTGGGCCCAGTCTGTGCTGACTCAGCCAC10800.12450687208763439No Hit
GTGCAGTGGGTGCGACAGGCTCGTGGACAACGCCTTGAGTGGATAGGATG10680.12312346239777179No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC10660.12289289411612803No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC10450.12047192715886847No Hit
GGTTATAAGGGAGACCTACTACTATGCTTTGGACGTCTGGGGCCAAGGGA10180.1173592553566776No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT10170.11724397121585571No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC10160.11712868707503382No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA9800.11297845800544602No Hit
CCCAGGAACGGCCCCCAAACTCCTCATCTCTAAGAATAATCAGCGGCCCT9780.11274788972380224No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG9640.11113391175229588No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA9530.10986578620325516No Hit
ATACTGTGCAGTGGGTGCGACAGGCTCGTGGACAACGCCTTGAGTGGATA9500.10951993378078952No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA9410.10848237651339256No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG9180.10583084127448923No Hit
CACCAGTGTGGCCCTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA9150.10548498885202358No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC9030.10410157916216098No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG9000.10375572673969533No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG8830.10179589634572331No Hit
GTATACCTAGTAAAGGTGAATCCAGAAGCCTTGCAGGAGACCTTCACTGA8820.10168061220490142No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAATACTAAGGC8780.10121947564161388No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA8690.10018191837421694No Hit
GTACTTATGTTTACTGGTACCAGCACCTCCCAGGAACGGCCCCCAAACTC8680.10006663423339505No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6750.081.222071
GTATCAA19100.073.271091
TCAACGC22150.060.1742174
ATCAACG22800.058.774723
CAACGCA22700.058.7094765
TATCAAC23900.056.672512
AACGCAG23950.055.8880426
TTACGGT450.00988064848.0309524
CGCAGAG27750.047.9728138
ACGCAGA29900.044.5232627
GCAGAGT31600.042.8111849
CAGCGCT3850.039.250489
GTACATG21500.032.881521
GAATACG2559.640644E-1131.0412339
CAGAGTA31400.030.7087129
ACATGGG22550.030.6716523
TACGGCC1204.2095556E-430.0193444
GTGCTAG2651.4733814E-1029.9440841
ATAGTAC3151.8189894E-1229.7334483
GCAGCGC5150.027.9453288
Produced by FastQC (version 0.11.5)