FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281405

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281405
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences645560
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTACATGGAGCTGAGCAGCCTGAGACCTGAGGACACGGCCATGTATTA25800.3996530144370779No Hit
ATGTATTACTGTGCGATTTTAGTAGAACCAACCGCCATCGTTGACTACTG21670.3356775512733131No Hit
GTAATACATGGCCGTGTCCTCAGGTCTCAGGCTGCTCAGCTCCATGTAGA21610.33474812565834317No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC15370.23808786170146848No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT14870.23034264824338557No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC13750.2129933700972799No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13450.20834624202243016No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA13270.20555796517752029No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC12590.19502447487452754No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC12550.1944048577978809No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12480.1933205279137493No Hit
GTATAGTAATGATCGGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCT12370.19161658095297107No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT12160.18836359130057623No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC11560.17906933515087675No Hit
GACCTAGGACGGTCAGCCTGGTCCCCCCGCCGAACACCCGACCATTCAGG11500.1781399095359068No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA10940.16946527046285395No Hit
CCATGTAGACTGTGCTCGTGGATTCGTCCGCGGTAATCGTGACTCTGCCC10520.16295929115806432No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10340.16017101431315448No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC10290.15939649296734618No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG9440.14622963008860526No Hit
TCCCTATCTTTCGTACAGCAAAGTACGCACAGAAGTTTCAGGGCAGAGTC9220.14282173616704877No Hit
GTATTACTGTGCGATTTTAGTAGAACCAACCGCCATCGTTGACTACTGGG9210.1426668318978871No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA9120.1412726934754322No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC9110.14111778920627052No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCCCCGCCGAACACCCG8900.1378647995538757No Hit
CATGTAGACTGTGCTCGTGGATTCGTCCGCGGTAATCGTGACTCTGCCCT8820.13662556540058243No Hit
GTACATGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATC8670.1343020013631576No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG8490.13151372451824772No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC8370.12965487328830783No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG8360.12949996901914618No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG8270.12810583059669126No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT8160.12640188363591298No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC8100.12547245802094306No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT8030.12438812813681144No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA7790.12067042567693166No Hit
GCCATGTATTACTGTGCGATTTTAGTAGAACCAACCGCCATCGTTGACTA7770.12036061713860834No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA7690.11912138298531508No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA7680.11896647871615341No Hit
TTACTATACATGAGGAGTTTGGGGGCCGTTCCTGGGAACTGCTGGTACCA7530.11664291467872856No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT7200.11153107379639382No Hit
CAGTAATACATGGCCGTGTCCTCAGGTCTCAGGCTGCTCAGCTCCATGTA7180.11122126525807052No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA7070.10951731829729226No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT7060.10936241402813061No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC6820.10564471156825081No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA6780.10502509449160419No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC6780.10502509449160419No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA6730.1042505731457959No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT6680.1034760517999876No Hit
CCTCTAAAGAAGCCCCTGGGAGCACAGCTCATCACCATGGACTGGACCTG6590.10208191337753268No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT6560.10161720057004771No Hit
GAAATAATCAGCCTCGTCCTCAGACTGGAGCCTAGTGATGGCCAGGGAGG6550.10146229630088606No Hit
ACATGGAGCTGAGCAGCCTGAGACCTGAGGACACGGCCATGTATTACTGT6470.10022306214759277No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC6460.10006815787843112No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14750.070.411911
ATCAACG15250.066.627613
CAACGCA15500.065.547895
TCAACGC15550.065.3421864
AACGCAG16300.062.2632066
TATCAAC16600.061.6432082
GGTATCA5400.061.438381
GAATACG2150.056.8996439
CGCAGAG18350.055.686688
GTGTACG400.00617587654.0924841
ACGCAGA20900.048.548067
GTGCTAG2400.048.082211
CAGCGCT3550.042.5686659
TATTGAG6950.041.4711885
GTATAGG1701.9226718E-938.182931
GTACATG18350.036.9461
GCAGCGC4700.035.215118
GCAGAGT30400.033.613519
TTATTGA8750.032.9425284
ATGGGAG8250.031.4427015
Produced by FastQC (version 0.11.5)