FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences645560
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTACATGGAGCTGAGCAGCCTGAGACCTGAGGACACGGCCATGTATTA23050.3570543404176219No Hit
GTAATACATGGCCGTGTCCTCAGGTCTCAGGCTGCTCAGCTCCATGTAGA21060.32622839085445193No Hit
ATGTATTACTGTGCGATTTTAGTAGAACCAACCGCCATCGTTGACTACTG18840.2918396431005639No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC13520.20943057190656172No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA13320.20633248652332856No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT12920.20013631575686225No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC12780.19796765598859906No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT12690.19657351756614413No Hit
GTATAGTAATGATCGGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCT11800.18278703761075654No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA11560.17906933515087675No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11420.17690067538261356No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC10990.17023979180866225No Hit
GACCTAGGACGGTCAGCCTGGTCCCCCCGCCGAACACCCGACCATTCAGG10700.16574756800297416No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC10630.16466323811884256No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC10360.1604808228514778No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA10210.1581572588140529No Hit
CCATGTAGACTGTGCTCGTGGATTCGTCCGCGGTAATCGTGACTCTGCCC9880.15304541793171822No Hit
GTATTACTGTGCGATTTTAGTAGAACCAACCGCCATCGTTGACTACTGGG9060.14034326786046225No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA9010.13956874651465395No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCCCCGCCGAACACCCG8980.13910403370716895No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG8840.13693537393890576No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC8810.13647066113142078No Hit
CATGTAGACTGTGCTCGTGGATTCGTCCGCGGTAATCGTGACTCTGCCCT8620.1335274800173493No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC8200.12702150071255963No Hit
TCCCTATCTTTCGTACAGCAAAGTACGCACAGAAGTTTCAGGGCAGAGTC8100.12547245802094306No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC8100.12547245802094306No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA7930.12283908544519487No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA7700.11927628725447673No Hit
TTACTATACATGAGGAGTTTGGGGGCCGTTCCTGGGAACTGCTGGTACCA7620.11803705310118348No Hit
GTACATGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATC7590.11757234029369851No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG7560.11710762748621353No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC7510.11633310614040523No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT7380.11431935064130368No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG7360.11400954210298035No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG7350.1138546378338187No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT7320.11338992502633373No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA7220.11184088233471715No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT7120.11029183964310058No Hit
CAGTAATACATGGCCGTGTCCTCAGGTCTCAGGCTGCTCAGCTCCATGTA6830.10579961583741249No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT6720.10409566887663424No Hit
GAAATAATCAGCCTCGTCCTCAGACTGGAGCCTAGTGATGGCCAGGGAGG6620.10254662618501766No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT6590.10208191337753268No Hit
GCCATGTATTACTGTGCGATTTTAGTAGAACCAACCGCCATCGTTGACTA6550.10146229630088606No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA6530.10115248776256273No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)