FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281407

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281407
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1232707
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGAGTCCTCAGATCTCAGGCTGCTCAACTCCATGTAGA35050.28433358454198765No Hit
TCTATAGGGATGGCAACCGGCCCTCTGGAATCCCTGAGCGATTCTCTGGC32130.2606458793533256No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC28640.23233420431619192No Hit
GTCTACATGGAGTTGAGCAGCCTGAGATCTGAGGACTCGGCCGTATATTA26180.21237812391752459No Hit
GGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTG23930.1941256113577679No Hit
GTATATTACTGTGCGAGAGATCTGTTCGGGGATTGTAGTGGCGAAAGATG23560.19112408707016348No Hit
CCTATGAACTGACTCAGCCACTCTCAGAGTCAGTGGCCCTGGGACAGATG20160.16354251253542001No Hit
GACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAACACCCAGCTGCTGTCC19850.16102772191607576No Hit
CCATGTAGACTGTGCTCGTGGATTCGTCCGCGGTAATCGTGAGTCTGCCC19800.16062211052585895No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAGGGTCTCCTGCA18890.1532399832239129No Hit
ATGTACAGGTGTCCAGCCCCAGGTGCAGCTGGTGCAGTCTGGGGCTGAGG17290.14026041873697478No Hit
CATGTAGACTGTGCTCGTGGATTCGTCCGCGGTAATCGTGAGTCTGCCCT15930.12922778892307743No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT15650.12695636513786324No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC15490.12565840868916944No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA15190.12322474034786855No Hit
CTTTACAGGTTCTGCGGCCTCCTATGAACTGACTCAGCCACTCTCAGAGT14790.11997984922613404No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAACACCCA14470.1173839363287464No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA14140.11470690115331543No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT13500.10951507535854017No Hit
CTCTCAGAGTCAGTGGCCCTGGGACAGATGGCCTGGATTACCTGTGAGGG13240.10740589612941276No Hit
CCCTATAGATGACCAACACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAG13060.1059456951246322No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)