FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281409

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281409
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences783820
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCCTGTCATTTGCAGATGCA35720.45571687377203945No Hit
GTGTATTACTGTGTGAGCCCACTTGATTCAAAAAATGATCCCTACTACTA30260.3860580235258095No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC23360.2980276083794749No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA22860.2916485927891608No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT22110.2820800694036896No Hit
GTATTACTGTGTGAGCCCACTTGATTCAAAAAATGATCCCTACTACTACT19760.2520986961292133No Hit
CTGTAGGACAGCTGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT19300.24623000178612434No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC18200.23219616748743335No Hit
CAGTAATACACAGCCGTGTCCTCGGCTCTCAGGCCTGTCATTTGCAGATG18000.2296445612513077No Hit
GACCTAGGACGGTCAACCTGGTCCCTCCGCCGAACACCCGCCGAGCACCA17600.22454134877905643No Hit
TACGTAGAGTACGCAGACTCAGTGAAGGGCCGATTCACCACCTCCAGAGA17230.219820877242224No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC16930.21599346788803553No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG14950.19073256615039166No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT14700.1875430583552346No Hit
TCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCCCCAAGGAG14430.18409838993646502No Hit
CTACTACGGCGTGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCT14350.18307774744201474No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGG14050.17925033808782628No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG13750.17542292873363782No Hit
CCTTCCCAGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG13530.1726161618738996No Hit
CTCCTGGACACCTGCCCGCTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTG13490.1721058406266745No Hit
ATACACAGCCGTGTCCTCGGCTCTCAGGCCTGTCATTTGCAGATGCAGTG13260.16917149345513No Hit
GCTGTGTATTACTGTGTGAGCCCACTTGATTCAAAAAATGATCCCTACTA12590.16062361256410912No Hit
CCGTAGTAGTAGTAGGGATCATTTTTTGAATCAAGTGGGCTCACACAGTA12390.15807200632798346No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT12380.1579444260161772No Hit
GCCTTGGGCTGACCTAGGACGGTCAACCTGGTCCCTCCGCCGAACACCCG12210.15577556071547038No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACA11870.15143783011405682No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGATTTGCCATGACTTGGG11800.15054476793141283No Hit
GTAATAGTCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAG11620.14824832231889973No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGATTTGCC11300.1441657523410987No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA11200.1428899492230359No Hit
GTGTGAGCCCACTTGATTCAAAAAATGATCCCTACTACTACTACGGCGTG11090.14148656579316682No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT11050.14097624454594168No Hit
GCCTGAGGATGAGGCTGACTATTACTGCTTGATCTCCGATAGTGGTGCTC10970.1399556020514914No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA10890.13893495955704116No Hit
TACTTACGTAGAGTACGCAGACTCAGTGAAGGGCCGATTCACCACCTCCA10830.13816947768620347No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAAC10770.13740399581536578No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT10660.13600061238549666No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCT10630.1356178714500778No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA10620.13549029113827155No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA10390.13255594396672704No Hit
GCTGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGTTCCACGACAC10280.13115256053685795No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC10240.13064223928963284No Hit
CTCTACGTAAGTACTACCACTAGTAATGGACGAGACCCACTCCAGCCCCT10150.12949401648337627No Hit
GTGTAGGTCTGGGTGCCGAAGTTGCTGGAGGGCACGGTCACCACGCTGCT10110.12898369523615116No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGG10100.12885611492434487No Hit
GTCTTAGCCCTGGATTCCAAGGCCTATCCACTTGGTGATCAGCACTGAGC9990.12745273149447578No Hit
GACTATTACTGCTTGATCTCCGATAGTGGTGCTCGGCGGGTGTTCGGCGG9900.12630450868821924No Hit
ACCTAGGACGGTCAACCTGGTCCCTCCGCCGAACACCCGCCGAGCACCAC9840.12553902681738155No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCTGGGAAGGTGTGCA9800.1250287055701564No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT9770.12464596463473757No Hit
GTACATGGGAGCTCTGAGAGAGGAGTCTTAGCCCTGGATTCCAAGGCCTA9750.124390804011125No Hit
GAGTACGCAGACTCAGTGAAGGGCCGATTCACCACCTCCAGAGACAACGC9710.12388048276389989No Hit
GCTCCAGGAGCACCTCCGAGAGCACAGCGGCCCTGGGCTGCCTGGTCAAG9710.12388048276389989No Hit
GTCTGGGGGAGGCCTGGTCAAGCCTGGGGGGTCCCTGAGACTCTCCTGTG9700.12375290245209358No Hit
ACGTAAGTACTACCACTAGTAATGGACGAGACCCACTCCAGCCCCTTCCC9650.12311500089306217No Hit
GATTTATGATACAACCAACAAACACTCCTGGACACCTGCCCGCTTCTCAG9540.12171161746319308No Hit
GTGTGAGGTTCAACTGGTGGAGTCTGGGGGAGGCCTGGTCAAGCCTGGGG9530.1215840371513868No Hit
CACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA9390.11979791278609886No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAG9390.11979791278609886No Hit
GTCCACCACCACGCACGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT9340.11916001122706744No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC9330.11903243091526115No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAA9300.11864968997984231No Hit
AGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTC9290.11852210966803603No Hit
GCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGGTTTTC9260.11813936873261718No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAAC9250.11801178842081089No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTG9240.11788420810900462No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG8870.11316373657217219No Hit
CTACTACTACGGCGTGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCT8820.11252583501314077No Hit
GTCCTGAGGACTGTAGGACAGCTGGGAAGGTGTGCACGCCGCTGGTCAGA8810.1123982547013345No Hit
GTCCTGGTGCACAACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGA8710.11112245158327166No Hit
GTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAAC8620.10997422877701514No Hit
CTACTACTACTACGGCGTGGACGTCTGGGGCCAAGGGACCACGGTCACCG8610.10984664846520883No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACAACGGTGAGGACGC8430.10755020285269579No Hit
CTGTTGAACTGCTCCTCCCGTGGCTTTGTCTTGGCATTATGCACCTCCAC8420.10742262254088948No Hit
GTACGCAGACTCAGTGAAGGGCCGATTCACCACCTCCAGAGACAACGCCA8270.10550891786379527No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8260.10538133755198897No Hit
GTCCAGGAGTGTTTGTTGGTTGTATCATAAATCAGTGTCCTGGGGGCTTG8200.10461585568115128No Hit
GTTCAACAGCACGTTCCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG8200.10461585568115128No Hit
GCCACAGGTGGTGCTGGGCACGGTGGGCACTCGACACAACATTTGCGCTC8160.10410553443392614No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA8110.10346763287489476No Hit
CCTCCAGAGACAACGCCAACAACTCACTGCATCTGCAAATGACAGGCCTG8060.10282973131586334No Hit
CCTGAGGACTGTAGGACAGCTGGGAAGGTGTGCACGCCGCTGGTCAGAGC8060.10282973131586334No Hit
TTTAGAAGGTGTCCAGTGTGAGGTTCAACTGGTGGAGTCTGGGGGAGGCC7970.10168150850960679No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA7930.10117118726238167No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT7920.10104360695057538No Hit
CTTACGTAGAGTACGCAGACTCAGTGAAGGGCCGATTCACCACCTCCAGA7840.10002296445612512No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5450.083.40341
GTATCAA12550.074.162731
CGTAATG300.001984071572.0492252
TATCGGT300.00199010871.994077
ATCAACG16050.056.967273
TATCAAC16600.055.1219982
TCAACGC16600.055.0798034
CAACGCA17450.052.3968245
AACGCAG18250.050.494476
CGCAGAG20200.045.6200038
GCAGAGT22900.040.2412269
CAGCGCT4050.037.330269
GTACATG17550.035.3558271
TACATGG18000.033.22272
ACATGGG18100.032.6160963
GTAATAC21500.029.1958081
GGGTTTA4750.028.8602221
CAGCCGT21600.028.6643059
ACAGCCG21800.028.4013298
TACACAG25550.027.3323865
Produced by FastQC (version 0.11.5)