FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281411

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281411
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences757271
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAAGCTGCTCATTTTCTGGGCATCCACCCGGGAATCCGGGGTCCCT34240.45214989085809437No Hit
ATATTATGCTAGTCCCTTCACTTTCGGCCCTGGGACCAAAGTGGATATCA32010.42270204457849303No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT31600.4172878665629609No Hit
GTTTTATACAGCTCCAACAATAAGAACTACTTAGCTTGGTATCAGCAGAA30680.4051389793085962No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23060.30451450009309744No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA17880.23611098272613107No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT17380.2295083266096285No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16400.2165671206212835No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15100.1994002147183769No Hit
AGCACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATTTCCAGAGA15040.1986078959843966No Hit
GCTTAGGAGACTGTCCTGGTTTCTGCTGATACCAAGCTAAGTAGTTCTTA15020.1983437897397365No Hit
AACCAGGACAGTCTCCTAAGCTGCTCATTTTCTGGGCATCCACCCGGGAA14620.19306166484653445No Hit
TTATTACTGTCAGCAATATTATGCTAGTCCCTTCACTTTCGGCCCTGGGA14540.19200523986789406No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC14310.18896801805430288No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14070.18579874311838168No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG14010.18500642438440135No Hit
GTATGTGCTACCACCACTACCACTAATAGCTGAGACCCACTCCAGCCCCT13230.17470628084265738No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12720.16797157160382478No Hit
GCATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCT12690.16757541223683464No Hit
CTGTAGGACAACTGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT12570.16599077476887403No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT12470.16467024354557352No Hit
GAAATATACGGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATTCA12450.1644061373009134No Hit
GTCTGAGAGCCGAGGACACGGCCGTATATTTCTGTGCGAGAGATGGGTGG12240.16163302173198235No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT12240.16163302173198235No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11760.1552944718601399No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC11680.1542380468814995No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG11160.14737128452033685No Hit
GTTTATTACTGTCAGCAATATTATGCTAGTCCCTTCACTTTCGGCCCTGG11150.1472392313980068No Hit
ATGCTAGTCCCTTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGA11110.1467110189086866No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGG11020.14552254080771612No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10810.14274942523878506No Hit
GTACATGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTT10270.13561855663296232No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC10100.13337365355335143No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA9990.13192106920772087No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9830.1298082192504401No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCA9610.12690305055917894No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9580.12650689119218878No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT9540.12597867870286858No Hit
ATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATTTCCAGAGACAATT9480.1251863599688883No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGG9400.12412993499024787No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9320.12307351001160746No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA9300.12280940376694738No Hit
ATATACGGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATTCAGCG9210.12162092566597692No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC9180.12122476629898675No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT9130.1205645006873365No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGC9100.12016834132034634No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA8970.11845165073005569No Hit
GTGTGAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGG8910.11765933199607537No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACA8800.11620674765044482No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA8640.11409389769316401No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG8610.11369773832617384No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8550.11290541959219355No Hit
CCTTCCCAGTTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG8550.11290541959219355No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT8550.11290541959219355No Hit
GTATATTTCTGTGCGAGAGATGGGTGGAGGGCGGATTGTAGTAGTGGAAG8510.11237720710287333No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTG8450.11158488836889305No Hit
ACTACAATCCGCCCTCCACCCATCTCTCGCACAGAAATATACGGCCGTGT8430.11132078212423294No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAACTATGCCATGAGCTGGG8330.11000025090093243No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTG8260.10907587904462207No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC8230.10867971967763192No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA8230.10867971967763192No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAAC8140.10749124157666146No Hit
GCCGTATATTTCTGTGCGAGAGATGGGTGGAGGGCGGATTGTAGTAGTGG8120.10722713533200134No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA8100.10696302908734126No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAAC8040.10617071035336095No Hit
GCGTAGTATGTGCTACCACCACTACCACTAATAGCTGAGACCCACTCCAG7980.10537839161938063No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAG7890.10418991351841017No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTAGCAACTATGCC7850.10366170102908998No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGA7830.10339759478442988No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC7770.10260527605044957No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC7730.10207706356112937No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCT7700.10168090419413922No Hit
CACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA7650.10102063858248897No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16250.062.614431
TCAACGC16650.060.964924
ATCAACG16800.060.420593
CAACGCA16850.060.2413065
TATCAAC17250.058.4618032
AACGCAG17600.057.6742026
CGCAGAG20400.049.7581338
GTGCTAG3000.045.7026141
GCAGAGT23450.043.5933889
TCTAGAC855.5671306E-542.347353
CAGCGCT3300.039.267549
GAATACG3400.038.1126179
ACATGGG18550.037.6446273
GTACATG18600.037.6328851
TACATGG18650.037.4650422
CTAGAGA3950.036.4508824
GTACACG7150.036.2469645
TACACGG7600.034.100766
GTGTATA2551.8189894E-1233.95861
GTATGTA8650.032.5354771
Produced by FastQC (version 0.11.5)