FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281412

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281412
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences757271
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT28150.3717295393590934No Hit
ATATTATGCTAGTCCCTTCACTTTCGGCCCTGGGACCAAAGTGGATATCA26670.3521856772542458No Hit
CTCCTAAGCTGCTCATTTTCTGGGCATCCACCCGGGAATCCGGGGTCCCT25040.33066101831444755No Hit
GTTTTATACAGCTCCAACAATAAGAACTACTTAGCTTGGTATCAGCAGAA23680.3127017936775606No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17500.23109296407758914No Hit
GCATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCT15890.20983241138245093No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT15710.20745545518051No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA15240.20124895843099763No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14890.19662709914944587No Hit
GCTTAGGAGACTGTCCTGGTTTCTGCTGATACCAAGCTAAGTAGTTCTTA13700.18091277759216978No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC12180.16084070299800202No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11990.15833169367373107No Hit
TTATTACTGTCAGCAATATTATGCTAGTCCCTTCACTTTCGGCCCTGGGA11960.1579355343067409No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11670.15410599375916945No Hit
GTATGTGCTACCACCACTACCACTAATAGCTGAGACCCACTCCAGCCCCT11630.15357778126984925No Hit
AACCAGGACAGTCTCCTAAGCTGCTCATTTTCTGGGCATCCACCCGGGAA10950.14459816895140576No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10900.1439379033397555No Hit
GTTTATTACTGTCAGCAATATTATGCTAGTCCCTTCACTTTCGGCCCTGG10820.14288147836111512No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGA10620.14024041591451408No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG10530.13905193781354364No Hit
GAAATATACGGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATTCA10510.13878783156888352No Hit
AGCACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATTTCCAGAGA10360.13680703473393277No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT10250.1353544503883022No Hit
ATGCTAGTCCCTTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGA9900.13073259110675042No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC9790.12928000676111986No Hit
GTCTGAGAGCCGAGGACACGGCCGTATATTTCTGTGCGAGAGATGGGTGG9720.1283556349048095No Hit
CTGTAGGACAACTGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT9570.12637483806985872No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA9420.12439404123490798No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG9340.12333761625626757No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT9220.12175297878830695No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9110.1203003944426764No Hit
GTCCTGGTGCACAACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGA9080.11990423507568626No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA9010.11897986321937588No Hit
GTACATGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTT8830.11660290701743498No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCA8620.1138297914485039No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGG8550.11290541959219355No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA8410.11105667587957284No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT8360.11039641026792259No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC8050.10630276347569101No Hit
ATATACGGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATTCAGCG8030.1060386572310309No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGG7960.10511428537472055No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT7920.10458607288540034No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGT7920.10458607288540034No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7830.10339759478442988No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT7690.10154885107180917No Hit
ATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATTTCCAGAGACAATT7660.10115269170481903No Hit
GTGTGAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGG7590.10022831984850865No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)