FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences706621
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC17330.24525169786915477No Hit
GCCTTGGGCTGACCGAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCCG17160.2428458820216212No Hit
GGTTAATAGTGATGGCAGCCACAACAAGGGGGACGGCATCCCTGATCGCT17120.24227980770455448No Hit
GTAATACAGGGCCGTGTCCTCAGATCTCAGGCTGCTCAGTTCCATGTAGG13300.1882197104246831No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA12270.17364329676021517No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA10650.15071728691901315No Hit
GTATTACTGTGCGAGAGAGGATGGTTACTATGATAGTCGTGGTTATTTCG10500.148594508230013No Hit
CTATTAACCTTCATCAAAAACCGAGGGCCCTTCTCTGGCCGCTGCTGATG10400.1471793224373462No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT10370.14675476669954615No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA10330.14618869238247945No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCC10220.14463198801054597No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA9750.13798061478501206No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT9680.1369899847301453No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT9290.1314707601387448No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGCGGTGACTCAATCGC8980.12708368418147775No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT8930.12637609128514438No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC8840.12510242407174427No Hit
GTAATAGTCAGCCTCATCTTCAGACTGGAGGCTGGAAATGGTGAGGTAGC8530.1207153481144772No Hit
CTGTATTACTGTGCGAGAGAGGATGGTTACTATGATAGTCGTGGTTATTT8510.12043231095594385No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA8500.12029079237667717No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG8420.11915864374254373No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT8290.1173189022120769No Hit
ATACAGGGCCGTGTCCTCAGATCTCAGGCTGCTCAGTTCCATGTAGGCAG8280.11717738363281023No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT8230.11646979073647683No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA8080.11434701204747666No Hit
GACTATTACTGTCAGACCTGGGGCACTGGCATTCGGGTCTTCGGCGGAGG7830.11080904756580968No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC7730.10939386177314289No Hit
GCCCTGTATTACTGTGCGAGAGAGGATGGTTACTATGATAGTCGTGGTTA7650.10826171313900945No Hit
GTACATGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCC7580.10727108308414271No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA7570.10712956450487601No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG7520.10642197160854264No Hit
TCCTCACCCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGCGGTG7520.10642197160854264No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC7510.10628045302927594No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA7460.10557286013294256No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT7430.10514830439514251No Hit
CAGTAATACAGGGCCGTGTCCTCAGATCTCAGGCTGCTCAGTTCCATGTA7330.10373311860247574No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT7330.10373311860247574No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG7110.10061970985860878No Hit
GTCTGGGGCTGAAGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA7110.10061970985860878No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)