FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281415

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281415
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1170925
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATATGCAGAGGCCAGGCCAGGCCCCTGTGCTGGTCATCTTTTATGAT119021.0164613446634072No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC53480.456732924824391No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATTACAAGATCACTG45640.3897773128082499No Hit
CCTATGAACTGACTCAGCCACCCTCAGTGTCAGTGGCCCCAGGAAAGACG44660.3814078613062323No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG44070.37636910989175226No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATTAC36540.3120609774323718No Hit
GCATATACCAGTGCACATTTTTATTTCCAATGTTGGGTCCCCCACAGGTA31180.26628520187031623No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA30980.26457715054337383No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT29700.2536456220509426No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC29310.250314921963405No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC26930.22998911117279075No Hit
GTACATGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCA26790.22879347524393107No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT26340.22495035975831074No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATTACAAGATCACTGT26190.22366932126310396No Hit
ATCCCACACCTGACAGAAATAGTCGGCCTCATCCCCGGCTCCGACCCCGC25720.21965540064478936No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA25540.21811815445054125No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG25010.21359181843414393No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCACCAACTCTGGGAACACGG24590.21000491064756496No Hit
CCCCTGTGCTGGTCATCTTTTATGATAATGACCGGCCCTCAGGGATCCCT24160.20633260029463887No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA23430.2000982129512992No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG23420.20001281038495208No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA23320.19915878472148088No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA22230.18984990498964494No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA22040.18822725622904968No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT21970.18762943826461986No Hit
GGCCAGGCCAGGCCCCTGTGCTGGTCATCTTTTATGATAATGACCGGCCC21900.18703162030019002No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC21580.1842987381770822No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGAACTGACTCAGCCAC21120.18037022012511475No Hit
GGCTCTGTGACCTCCTATGAACTGACTCAGCCACCCTCAGTGTCAGTGGC21010.17943079189529645No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT20830.17789354570104832No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT20400.1742212353481222No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT20350.17379422251638663No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA20280.17319640455195678No Hit
GGCCAGGCCCCTGTGCTGGTCATCTTTTATGATAATGACCGGCCCTCAGG19190.16388752482012084No Hit
CTGTTACTATCCCACACCTGACAGAAATAGTCGGCCTCATCCCCGGCTCC18980.16209407092683134No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC18840.16089843499797168No Hit
GCACAGGCTCTGTGACCTCCTATGAACTGACTCAGCCACCCTCAGTGTCA17880.1526997886286483No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC17630.15056472446997032No Hit
TAGTAACAGTGATCTTGTAATATTCGGCGGAGGGACCAAGCTGACCGTCC17150.1464654012853086No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG16800.14347631146315948No Hit
ATGCAGAGGCCAGGCCAGGCCCCTGTGCTGGTCATCTTTTATGATAATGA16640.14210987040160558No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG16590.14168285756986998No Hit
GTGTGGGATAGTAACAGTGATCTTGTAATATTCGGCGGAGGGACCAAGCT16530.14117044217178726No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAG16270.1389499754467622No Hit
GTATCAACGCAGAGTACATGGGAGTCTGGGCCTAAGGAAGCAGCACTGGT15650.1336550163332408No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15540.13271558810342252No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG15440.1318615624399513No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG15280.13049512137839742No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC15240.13015351111300894No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT14690.1254563699639174No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA14550.12426073403505776No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG14400.12297969553985097No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGT14340.12246728014176826No Hit
GGATTACCTGTGGGGGACCCAACATTGGAAATAAAAATGTGCACTGGTAT14300.1221256698763798No Hit
CCCTCAGTGTCAGTGGCCCCAGGAAAGACGGCCAGGATTACCTGTGGGGG14200.12127164421290859No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGG14140.12075922881482588No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT14090.1203322159830903No Hit
GTGCTGGTCATCTTTTATGATAATGACCGGCCCTCAGGGATCCCTGAGCG13710.11708691846189978No Hit
GCACAGGGGCCTGGCCTGGCCTCTGCATATACCAGTGCACATTTTTATTT13670.1167453081965113No Hit
CCCTGAGCATCAGCGGGGTCGGAGCCGGGGATGAGGCCGACTATTTCTGT13100.11187736191472554No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13070.11162115421568418No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT12960.11068172598586588No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12460.10641159766850994No Hit
CTCACTGCACAGGCTCTGTGACCTCCTATGAACTGACTCAGCCACCCTCA12420.10606998740312146No Hit
TCTTTTATGATAATGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGC12140.10367871554540213No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGG12130.10359331297905502No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC11980.10231227448384823No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTC11720.10009180775882316No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20000.078.763571
ATCAACG24400.063.899573
TCAACGC24650.063.221784
CAACGCA25250.062.0025945
TATCAAC25950.060.9278952
AACGCAG27200.057.812976
CGCAGAG32250.048.9817248
GGTATCA12200.048.7136841
ACGCAGA33400.046.8672147
GCAGAGT36850.042.856339
GTACATG30250.039.2930221
ATACGGG750.001586013538.144333
TATTGAG17400.037.3868875
TACATGG31350.037.1972922
ACATGGG32000.036.4308173
CATGGGA17250.033.5678184
TTATTGA19250.033.42254
GAATACG1958.052666E-932.9813279
TACGGGT900.003887270331.7720074
ACCCGTA900.0038897131.7679357
Produced by FastQC (version 0.11.5)