FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281416

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281416
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1170925
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATATGCAGAGGCCAGGCCAGGCCCCTGTGCTGGTCATCTTTTATGAT115220.9840083694515019No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC50920.4348698678395286No Hit
CCTATGAACTGACTCAGCCACCCTCAGTGTCAGTGGCCCCAGGAAAGACG41020.35032132715588105No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATTACAAGATCACTG40870.34904028866067427No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG40160.3429767064500288No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATTAC35170.30036082584281654No Hit
GCATATACCAGTGCACATTTTTATTTCCAATGTTGGGTCCCCCACAGGTA33690.287721246023443No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC30790.26295450178277857No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA28810.24604479364604906No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT27800.23741913444499008No Hit
GTACATGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCA25980.22187586736981446No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC25250.2156414800264748No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCACCAACTCTGGGAACACGG24170.206418002860986No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT23990.20488075666673786No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG23510.20078143348207617No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA22770.19446164357238935No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA22370.1910455409185046No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA22150.189166684458868No Hit
CCCCTGTGCTGGTCATCTTTTATGATAATGACCGGCCCTCAGGGATCCCT21720.18549437410594188No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATTACAAGATCACTGT21250.18148045348762729No Hit
ATCCCACACCTGACAGAAATAGTCGGCCTCATCCCCGGCTCCGACCCCGC20940.17883297393086664No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT20790.17755193543565984No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT20780.17746653286931274No Hit
GGCCAGGCCAGGCCCCTGTGCTGGTCATCTTTTATGATAATGACCGGCCC20760.17729572773661847No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA20400.1742212353481222No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT20370.17396502764908084No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG20340.1737088199500395No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC19500.16653500437688154No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA19330.16508316074898052No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGAACTGACTCAGCCAC19260.16448534278455068No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA19230.16422913508550932No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC19200.16397292738646796No Hit
GGCTCTGTGACCTCCTATGAACTGACTCAGCCACCCTCAGTGTCAGTGGC19060.1627772914576083No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT18490.15790934517582253No Hit
GGCCAGGCCCCTGTGCTGGTCATCTTTTATGATAATGACCGGCCCTCAGG17780.1518457629651771No Hit
TAGTAACAGTGATCTTGTAATATTCGGCGGAGGGACCAAGCTGACCGTCC17530.14971069880649915No Hit
CTGTTACTATCCCACACCTGACAGAAATAGTCGGCCTCATCCCCGGCTCC17200.14689241411704423No Hit
GTGTGGGATAGTAACAGTGATCTTGTAATATTCGGCGGAGGGACCAAGCT16470.14065802677370456No Hit
GCACAGGCTCTGTGACCTCCTATGAACTGACTCAGCCACCCTCAGTGTCA16440.1404018190746632No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC16390.1399748062429276No Hit
ATGCAGAGGCCAGGCCAGGCCCCTGTGCTGGTCATCTTTTATGATAATGA15710.13416743173132353No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG15440.1318615624399513No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG14920.12742062898990114No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG14790.1263103956273886No Hit
GGATTACCTGTGGGGGACCCAACATTGGAAATAAAAATGTGCACTGGTAT14790.1263103956273886No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG14710.12562717509661164No Hit
CCCTCAGTGTCAGTGGCCCCAGGAAAGACGGCCAGGATTACCTGTGGGGG14240.12161325447829707No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA14200.12127164421290859No Hit
GTATCAACGCAGAGTACATGGGAGTCTGGGCCTAAGGAAGCAGCACTGGT14060.12007600828404894No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13670.1167453081965113No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG13630.11640369793112283No Hit
GCACAGGGGCCTGGCCTGGCCTCTGCATATACCAGTGCACATTTTTATTT13240.11307299784358518No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGG13180.11256058244550249No Hit
GTGCTGGTCATCTTTTATGATAATGACCGGCCCTCAGGGATCCCTGAGCG13130.1121335696137669No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT13020.11119414138394859No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAG12960.11068172598586588No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT12780.10914447979161773No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC12770.10905907722527063No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGT12470.10649700023485704No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA12360.10555757200503876No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA12170.10393492324444349No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGG11850.1012020411213357No Hit
CCCTGAGCATCAGCGGGGTCGGAGCCGGGGATGAGGCCGACTATTTCTGT11840.10111663855498858No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12750.069.469131
TCAACGC14900.058.478474
ATCAACG15150.057.0381623
TATCAAC16050.054.2884252
CAACGCA17200.050.6586765
AACGCAG18100.048.9208076
CGCAGAG20300.041.8459138
GTACATG30650.039.9406741
TACATGG30650.039.0008932
ACATGGG32200.036.6762473
GCAGAGT23950.035.468569
ACGCAGA24250.035.0297747
GGTATAT52950.034.6793631
CATGGGA18150.034.5175174
TAGGGCG1051.9291663E-434.29085
TATATGC55450.032.856093
GGTATCA9650.032.8338851
TATTGAG19450.032.2104345
TATGCAG57100.032.1588825
GTATATG57250.031.5714932
Produced by FastQC (version 0.11.5)