FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281417

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281417
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences782223
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAATACACAGCCGTGTCTGCGGCGGTCACAGAGTTCACGTTCAGGGAGA18650.23842305838616354No Hit
GTCTCTGGTGGCTCCATCAGCAGGAGTCATTACTTCTGGGTCTGGATTCG14260.18230095509848215No Hit
GTGTATTATTGTGCGAGTATTGTGGGAGCCATTCTTGAAGACTGGGGCCA12980.16593733500549077No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT11560.14778394396482844No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA11520.14727258083692246No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11490.14688905849099299No Hit
GTATTATTGTGCGAGTATTGTGGGAGCCATTCTTGAAGACTGGGGCCAGG11170.14279815346774513No Hit
CAATAATACACAGCCGTGTCTGCGGCGGTCACAGAGTTCACGTTCAGGGA10970.14024133782821524No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT9980.1275851004125422No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT9870.12617885181080074No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC9140.11684647472651662No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT8890.11365045517710423No Hit
GTCCCTCAACTGCGCTGTCTCTGGTGGCTCCATCAGCAGGAGTCATTACT8760.1119885250114098No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18050.054.407351
TCAACGC19950.047.9952054
ATCAACG20300.047.5223433
CAACGCA20950.045.7042665
AACGCAG21450.045.310166
TATCAAC23150.041.725212
CGCAGAG23700.041.008568
ACGCAGA27450.035.1440287
GCAGAGT28150.034.5258569
GTACACG6050.030.9390585
TACACGG6450.029.0203596
ACACGGG6600.028.3608047
GTATGTA8000.027.0786551
GGTATCA13800.026.1629541
GTATATA4850.025.3106331
TATGTAC8350.023.3089122
ACGGGGT8450.022.1516349
GTACATG22400.021.9208181
CTTACAC5950.021.7793373
CAGAGTA28800.021.7478289
Produced by FastQC (version 0.11.5)