FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281419

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281419
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences982130
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAATAGACAGCCGTGTCTTCAGATCTCAGGCTACTCAGCTCCATGTAGG25950.26422164071966037No Hit
GTCTATTATTGTGCGAGATCTCAGTATAGTCGCTCGTCGGAGTACAACTG21810.2220683616221885No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17180.17492592630303525No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15710.15995845763799088No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC15280.1555802185046786No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15250.15527475996049403No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA14630.14896195004734608No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT14050.14305641819311088No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC13530.13776180342724487No Hit
GGTCCAGGTTGTGCAGTCTGGGGCTGAAATGAAGAAGCCTGGGGCCTCAG13330.13572541313268102No Hit
GTCTGGGGCTGAAATGAAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCC13330.13572541313268102No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11590.11800881756997546No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC11460.11668516387850895No Hit
GCCTACATGGAGCTGAGTAGCCTGAGATCTGAAGACACGGCTGTCTATTA11460.11668516387850895No Hit
CTTCTGGGTACACCTTCACTGCCTTTGCTATCCATTGGGTGCGCCAGGCC11360.11566696873122702No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG11200.11403785649557595No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10380.10568865628786414No Hit
CTATTATTGTGCGAGATCTCAGTATAGTCGCTCGTCGGAGTACAACTGGT10120.10304134890493112No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA9920.10100495861036726No Hit
GTATTACTGTCAGCTGTATGGTATCTCACCGTACCCTTTTGGCCAGGGGA9910.10090313909563906No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16650.072.269321
TCAACGC17250.067.962964
CAACGCA17850.066.078975
AACGCAG18650.063.2219476
TATCAAC19350.061.000072
GGTATCA6450.054.412071
CGTACGC400.006379718453.6523322
CGCAGAG23050.051.153558
ACGCAGA25700.045.8789637
ATCAACG30950.039.051953
GTACATG22000.033.2075161
TAGACGG7400.030.9016046
GTAGACG7450.030.7051545
ACATGGG23200.028.977213
GCAGAGT42250.028.41349
TAGACAG16700.027.3956175
CATGGGA14050.026.9660364
ATAGACA17050.026.8332424
AATAGAC15250.026.2624263
CAGCCGT14800.026.0718989
Produced by FastQC (version 0.11.5)