FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281421

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281421
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences742644
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG24340.3277478845853464No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG22360.30108638863304626No Hit
GTATACGGCTGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAGATAGGCGA21970.29583488185456286No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG18370.24735943466856258No Hit
GCCGTATACTACTGTACTAGATCGATTTTTTGGAGCGGTTATTATGACCA17540.23618315101179033No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT16720.22514152137497914No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT14090.1897275141252067No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG13880.18689977970602334No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC13220.17801261438858995No Hit
CTCCTGTACAGCTTCTGGGTTCACCTTTGGTACTTATGCTATGAGCTGGT13110.1765314201690177No Hit
GTAGTATACGGCTGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAGATAGG12860.17316506966998993No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT10940.14731149783745645No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC10290.13855898653998416No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT9660.13007578328243413No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9300.12522823856383408No Hit
GTATACTACTGTACTAGATCGATTTTTTGGAGCGGTTATTATGACCAGTA8510.11459057098690624No Hit
CTACTGTACTAGATCGATTTTTTGGAGCGGTTATTATGACCAGTATTTCT8320.11203214460764513No Hit
GTACAGTAGTATACGGCTGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAG8110.10920441018846176No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC8050.10839648606869509No Hit
CCCTGAGACTCTCCTGTACAGCTTCTGGGTTCACCTTTGGTACTTATGCT7880.1061073677293562No Hit
GACCTAGGACGGTCAGTATGGTCCCTCCGCCGAACACCAAGGGATCACTA7860.10583805968943398No Hit
GTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTC7540.10152913105067839No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)