FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281422

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281422
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences742644
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG20990.28263878789837393No Hit
GTATACGGCTGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAGATAGGCGA18810.25328421154685155No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG18670.2513990552673959No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG15180.20440480230096789No Hit
GCCGTATACTACTGTACTAGATCGATTTTTTGGAGCGGTTATTATGACCA13570.18272550508722887No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT12890.17356903172987326No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT12580.1693947571110788No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT11580.1559293551149676No Hit
CTCCTGTACAGCTTCTGGGTTCACCTTTGGTACTTATGCTATGAGCTGGT10990.147984767937262No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC10870.14636891969772864No Hit
GTAGTATACGGCTGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAGATAGG10480.1411174129192453No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC9420.12684408680336742No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG9370.12617081670356187No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC9060.12199654208476739No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT8060.1085311400886562No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7550.058.2428171
ATCAACG7250.056.5916753
TCAACGC7550.054.3430024
CAACGCA7900.051.93545
AACGCAG8500.048.269376
TATCAAC9500.045.4614562
ACGCAGA9400.044.413597
CGCAGAG10050.040.824848
CCGTATA6150.039.7941742
CGTATAC6300.037.7041473
ATAGGTG10450.036.5069274
TAGGTGG11250.035.8303685
GTCATAG11450.034.6271481
GGTATCA4800.034.5418931
CATAGGT12250.033.4930343
GTACATG15100.032.463211
GCCGTAT7500.031.7184681
TAATACG1153.297446E-431.2959334
TCATAGG12900.031.247412
TACATGG15850.030.8812872
Produced by FastQC (version 0.11.5)