FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281423

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281423
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences849353
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTACGGAATGGACGTCTGGGGCCAAGGGACCACGGTCATCGTCTCCT24840.2924579061944798No Hit
GTCTGGGGCTGAGGTGAGGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGTA17400.2048618183487902No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16410.1932058873048073No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC15330.18049032616591687No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA15170.17860653933052573No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT14890.17530991236859114No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA14590.1717778120522327No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC14280.16812797505866228No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13470.15859130420449447No Hit
CTCCTGGACCCCTGCCCGATTCTCAGGCTCCCTCCTTGGGGGCAAAGCGG13260.15611883398304358No Hit
CATTTATAGTACATACAACAGACACTCCTGGACCCCTGCCCGATTCTCAG11830.13928248914173494No Hit
GTATATTACTGCCTGCTCTACTATGGTGATGCTCGGGTCTTCGGAACTGG11560.13610359885701234No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG11530.1357503888253765No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG11350.13363112863556142No Hit
CCGTAGTACTGTCCGCCGGGCTGAAGGGGACTCGCACAATAGTACACGGC11050.13009902831920298No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10950.12892166154708348No Hit
GTGTACTATTGTGCGAGTCCCCTTCAGCCCGGCGGACAGTACTACGGAAT10910.1284507148382357No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG9730.11455778692722578No Hit
ATATTCTGGGTGCGACAGGCCCCTGGACAAGGGCTAGAGTGGATGGGAGG9650.11361589350953019No Hit
GTACTATTGTGCGAGTCCCCTTCAGCCCGGCGGACAGTACTACGGAATGG9610.11314494680068239No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA9270.10914189977547617No Hit
GTAATATACAGCCTCGTCCTCAGGCAGCACACCTGACAGTGTCAGGGCCG9100.10714037626287304No Hit
CCTCTAAAGAAGCCCCTGGGAGCACAGCTCATCACCATGGACTGGACCTG9090.10702263958566108No Hit
CCTGTAAGGCCTCTGGAGGCACCTTCAGCGCCCGCACTATATTCTGGGTG9080.10690490290844915No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC8860.10431469600978628No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG8830.10396148597815044No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT8560.10078259569342783No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11150.075.14621
TCAACGC13350.062.1454664
ATCAACG13400.061.9573863
CAACGCA14050.059.049255
TATCAAC14300.058.5861052
AACGCAG15050.054.644056
GGTATCA5550.054.194141
CGCAGAG16800.048.951968
ACGCAGA20400.040.313387
GCAGAGT21550.038.464479
GTACATG19900.038.1459851
ACATGGG20250.036.404253
TACATGG20650.035.715932
TCTATAC2259.094947E-1031.8095383
ATAGGAC1652.6381185E-630.3636513
TCTACAC3800.030.1353533
CATGGGA11250.029.2440854
GACCGCG1255.583824E-428.6049737
CAGCGCT5600.028.072679
TCTAGAC2208.3746454E-726.0259863
Produced by FastQC (version 0.11.5)