FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281426

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281426
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences812598
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATGTACTGACTCAGCCACCCTCAGTGTCAGTGGCCCCAGGAAAGACG24190.29768717127041905No Hit
CTACATGGACTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTTG21200.2608916093812685No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC20090.24723171851272094No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT18060.2222501162936655No Hit
ATAATATACGGCCGTGTCCTCGATTTTCAGGCTGTTCAGTTGCAGGTACA17870.21991193677562584No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA17170.21129759118284808No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG16680.2052675492679037No Hit
GACCTAGGACGGTCAGCTTGGTCTCTCCGCCGAACAGCCAAAGATCACCA16410.20194487311068943No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC16330.20096037647151485No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC16240.19985281775244337No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA15930.1960378932756418No Hit
CTCTAGCACAATAATATACGGCCGTGTCCTCGATTTTCAGGCTGTTCAGT15680.19296134127822123No Hit
GTGCTAGAGAGGGGGTTTGTAGTGGTGCCAGCTGCTATCCCTCAGACTAC15220.18730048560296728No Hit
GTATATTATTGTGCTAGAGAGGGGGTTTGTAGTGGTGCCAGCTGCTATCC14790.18200881616740383No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14760.18163962992771332No Hit
ATATACGGCCGTGTCCTCGATTTTCAGGCTGTTCAGTTGCAGGTACAAGA14750.1815165678478165No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC14530.17880920209008638No Hit
GTGTTGGTCATGTATAATGATAGCGACCGGCCCTCAGGGATCCCAGAGCG14010.1724099739354515No Hit
GCCGTATATTATTGTGCTAGAGAGGGGGTTTGTAGTGGTGCCAGCTGCTA13420.1651493112215388No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCTCTCCGCCGAACAGCCA13410.165026249141642No Hit
GTACTGACTCAGCCACCCTCAGTGTCAGTGGCCCCAGGAAAGACGGCCAG12960.15948845554628488No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12670.15591965522927695No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT12280.1511202341133008No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT11810.14533631635815003No Hit
TGTATAATGATAGCGACCGGCCCTCAGGGATCCCAGAGCGATTCTCTGGC11750.1445979438787691No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA11670.1436134472395945No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG11550.14213670228083258No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC11420.14053689524217386No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC11190.1377064674045469No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA11030.13573747412619772No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA10950.1347529774870231No Hit
GTATAATGATAGCGACCGGCCCTCAGGGATCCCAGAGCGATTCTCTGGCT10850.13352235668805484No Hit
ATATTATTGTGCTAGAGAGGGGGTTTGTAGTGGTGCCAGCTGCTATCCCT10660.13118417717001518No Hit
GTACATGGGAGCTCTGAGAGCGGAGCCCCAGCCCCAGAATTCCCAGGTGT10650.13106111509011836No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT10330.12712312853341998No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG10220.1257694456545549No Hit
GTACATGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCA10200.12552332149476125No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT9880.12158533493806287No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT9840.12109308661847555No Hit
ATCCCACACCTGACAATAATAGTCGGCCTCGTCCCCGGCTTCGACCCTAC9780.12035471413909461No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT9650.11875490710043589No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA9440.11617060342260256No Hit
TTACTATCCCACACCTGACAATAATAGTCGGCCTCGTCCCCGGCTTCGAC9360.11518610678342797No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT9190.11309405142518195No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA9180.11297098934528513No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTACTGACTCAGCCAC9180.11297098934528513No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC9080.11174036854631687No Hit
CTCCTGTGCAGCCTCCGGATTCACCTTCAGTGACCACTACATGGACTGGG8920.10977137526796768No Hit
GCCCTCAGGGATCCCAGAGCGATTCTCTGGCTCCAACTCTGGGAACACGG8920.10977137526796768No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC8830.10866381654889626No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC8740.10755625782982485No Hit
ACCTAGGACGGTCAGCTTGGTCTCTCCGCCGAACAGCCAAAGATCACCAT8660.10657176119065023No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG8530.10497195415199152No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG8500.10460276791230104No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT8460.10411051959271374No Hit
CTACAAACCCCCTCTCTAGCACAATAATATACGGCCGTGTCCTCGATTTT8330.10251071255405501No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC8330.10251071255405501No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG8270.10177234007467408No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT8220.10115702967518994No Hit
GTGTGAGGTGCATCTGGTGGAGTCGGGGGGAGGCTTGGTCCAGCCTGGAG8130.10004947095611852No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)