FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281427

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281427
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences992602
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT31140.31372090727199825No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24970.2515610486378226No Hit
TTACTACTGCATGCAAGGTCTAAAAATTCCGTACACTTTTGGCCAGGGGA19220.1936324931845795No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16920.17046107100328228No Hit
CCCCTGGAGAGTCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCTTG16290.1641141162318835No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15440.1555507645561867No Hit
ATAATAGACAGCCGTGTCCTCGGCCCTCAGACTATTCATTTGCAGATACA15140.15252840514123486No Hit
GTCTAAAAATTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA15040.15152095200291757No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14710.14819635664647057No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14420.1452747425453505No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13940.14043896748142762No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12860.1295584735876011No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12600.12693909542797618No Hit
CAGTAGTAAATCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGT11800.11887947032143799No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT11570.11656232810330829No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11420.11505114839583236No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA11070.11152506241172191No Hit
TTTTTAGACCTTGCATGCAGTAGTAAATCCCAACATCCTCAGCCTCCACT10320.10396916387434238No Hit
GTAGTAAATCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAA10270.10346543730518376No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC10060.10134978571471748No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8950.070.899351
GTATCAA20250.069.43721
TCAACGC22750.060.8528024
ATCAACG22850.060.5864833
CAACGCA24050.057.563465
AACGCAG25050.055.251586
TATCAAC26100.053.594712
ACGCAGA28400.048.7022937
CGCAGAG29450.047.1819578
GTACATG24950.028.322931
TACATGG24450.028.0159592
CCGACCG2853.5106495E-1027.7876479
TAGACAG13600.027.5692635
ACCGCTC1856.1406517E-627.2414518
GCAGAGT54450.027.2378779
ACATGGG26600.026.5647143
ATAGACA13400.026.3664724
ACAGCCG11000.026.1800968
CAGCCGT11150.025.8278989
CAGAGTA34900.025.3736889
Produced by FastQC (version 0.11.5)