FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281430

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281430
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences797657
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCCTCAGTGCTCAGGCTGTTCATTTGCAGATAGA37240.4668673377153338No Hit
GTATATTACTGTGCGAGAGGGGGGTCAAATTCTTTGGTGTTAATTCCTAT29390.36845410997458805No Hit
TCATAATACTGTTGACAGTAATATGTTGCAAAATCTTCAGGGAGCAGGCT18860.2364424809159827No Hit
GTATTATGATACCCCGCTCACTTTCGGCGGCGGGACCAAGGTGGAGATCA17750.22252672514627211No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT14810.1856687774319037No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCACCTGGTGGAGTCTGGGGGAGGCG14450.18115555934443No Hit
ATATACAGCCGTGTCCTCAGTGCTCAGGCTGTTCATTTGCAGATAGAGGG13670.1713769201549037No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC13090.16410562434730716No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA12340.15470308666507032No Hit
GCTGTATATTACTGTGCGAGAGGGGGGTCAAATTCTTTGGTGTTAATTCC11970.15006450140850014No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA11390.14279320560090364No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT10620.13313993358047382No Hit
ATGATACCCCGCTCACTTTCGGCGGCGGGACCAAGGTGGAGATCAAACGA10580.13263846490408784No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC10200.1278745124784212No Hit
ATATTACTGTGCGAGAGGGGGGTCAAATTCTTTGGTGTTAATTCCTATTT10150.1272476766329387No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG10080.12637010644926328No Hit
GCTATGCACTGGGTCCGCCAGACTCCAGGCAAGGGGCTGGAGTGGGTGGC10040.12586863777287732No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC9790.12273445854546502No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT9630.12072858383992116No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT9590.12022711516353521No Hit
GACCAGGGAGAGCCCCTAAACTCCTTCTCTATGCAGCATCCAGATTGCAA9280.11634073292154395No Hit
GGGTATCATAATACTGTTGACAGTAATATGTTGCAAAATCTTCAGGGAGC9180.11508706123057905No Hit
CAGTAATATACAGCCGTGTCCTCAGTGCTCAGGCTGTTCATTTGCAGATA8990.11270508501774572No Hit
GTCTAAATAATATTTATTAATTCCATCATATGCTATAACTGCCACCCACT8890.11145141332678081No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT8600.10781576542298256No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT8150.10217424281364046No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC8010.10041910244628957No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC9900.050.920984
GTATCAA10300.050.3481671
ATCAACG10100.049.9126473
CAACGCA11050.045.6215135
TATCAAC11750.042.9063262
AACGCAG11750.042.8901826
CGCAGAG12350.040.8064468
CAGCCGT13900.039.88179
AATATAC15650.037.273883
GTACATG20000.037.093311
TATACAG16450.036.7745445
GGCAGCG4350.036.4108737
GAATACG1251.3268862E-534.5572289
TACATGG20850.033.8517532
ATACAGC17600.033.133716
ACATGGG21150.033.0289883
ATATACA18650.032.8226784
TAATATA17500.032.1009332
GTATTAG4100.031.6211031
GCAGAGT15700.031.1822159
Produced by FastQC (version 0.11.5)