FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281431

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281431
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences448867
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA22560.5025987653358345No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG15420.34353160290241874No Hit
GTATATTACTGTGCGAAGGAAGGGTTGGGATGGAGTGGTTACCAAGGGTT11900.26511193738902616No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG11480.25575504548117817No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT9470.2109756342079057No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG9420.20986171850459046No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT9220.2054060556913295No Hit
ATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACATCA8440.1880289707196118No Hit
GTATTACTGTCAGCACTATGGTAGCTCACCTCCGAGAATGTACACTTTTG7710.17176580145120937No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC7590.1690924037632528No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG7430.16552787351264406No Hit
GTATGTGCTGCTACCACCGCTGTGAATAACTGAGACCCACTCCAGCCCCT7250.1615177769807092No Hit
GTGTGAGGTACAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCGGGGG7130.15884437929275266No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6910.1539431501981656No Hit
GCATAGTATGTGCTGCTACCACCGCTGTGAATAACTGAGACCCACTCCAG6790.15126975251020905No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT6730.14993305366623075No Hit
ATACTATGCAGACTCCGTGAAGGGCCGGTTCACCCTCTCCAGAGATAATT6690.14904192110357856No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT6100.13589771580445878No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT6040.1345610169604805No Hit
TCTCTGGAGAGGGTGAACCGGCCCTTCACGGAGTCTGCATAGTATGTGCT5950.13255596869451308No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC5730.12765473959992604No Hit
GTACATGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTT5720.127431956459263No Hit
CTCCAGAGATAATTCCAGGAACATGATGTATCTGCAAATGAACAGCCTGA5700.1269863901779369No Hit
GCCGTATATTACTGTGCGAAGGAAGGGTTGGGATGGAGTGGTTACCAAGG5670.12631804075594774No Hit
GGACTGAACAGAGAGGACTTACCATGGAGTTTGGGCTGAGCTGGCTTTTT5540.12342185992732814No Hit
CCCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTGGTCAAACCCTT5370.11963454653605633No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGCTATGCCATGAACTGGG5340.11896619711406721No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG5290.11785228141075196No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACT5080.11317383545682796No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGTTGATGGTGAGAGT5080.11317383545682796No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC5020.11183713661284968No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGCTATGCC4900.10916373892489312No Hit
TTTAAAAGGTGTCCAGTGTGAGGTACAGCTGTTGGAGTCTGGGGGAGGCT4790.1067131243775996No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC4690.10448529297096913No Hit
GTCTGCATAGTATGTGCTGCTACCACCGCTGTGAATAACTGAGACCCACT4580.10203467842367561No Hit
CAGTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA4550.10136632900168646No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC4510.10047519643903428No Hit
CTATGCAGACTCCGTGAAGGGCCGGTTCACCCTCTCCAGAGATAATTCCA4490.10002963015770817No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3400.080.502481
GTATCAA8600.065.32821
ATCAACG9750.056.8587233
TCAACGC9800.056.5686264
TATCAAC10050.055.161452
CAACGCA10200.054.350255
AACGCAG10550.053.2295956
ACACTCG450.00990469347.9976275
ACGCAGA12200.046.6206447
CGCAGAG12500.045.501758
ATAGGTG6200.042.9656144
GTATTGG1202.073757E-742.0166441
TAGGTGG6600.040.3616375
GTCATAG6650.040.0760231
CCGTTGA2950.039.0489129
GCAGAGT14650.038.8240179
CCCGTTG3150.036.569628
TCATAGG7350.036.2431072
GGTGGTC7500.035.518247
GTACATG10400.035.3216821
Produced by FastQC (version 0.11.5)