FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1385721
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATACA48930.35310138187990225No Hit
GTGTATTACTGTGCGAGACGTATAGAAGCAGCTGGTACTGGGGCTGAAAA44650.32221493359774445No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC40590.29291610648896854No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAACTGGTGGAGTCTGGGGGAGGCG33600.24247305193469681No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC29130.2102154762755273No Hit
AATAAATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGA28310.20429797917474007No Hit
GTATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGA27650.19953511565459428No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT26900.19412277074533762No Hit
GGTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAA26850.19376194775138716No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT26750.1930403017634863No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA26730.19289597256590613No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC26320.1899372240155125No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT26100.18834960284213056No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG25820.1863289940760081No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC25720.18560734808810722No Hit
GATTATTACTGCAGCTCATATACAGGCAGCAGCACTTTGGTGTTCGGCGG25670.18524652509415676No Hit
GCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGC25610.18481353750141624No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCAAAGTGCTGCTG25010.18048366157401094No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA24840.17925686339457944No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC24840.17925686339457944No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT24620.17766924222119748No Hit
GTATAGAAGCAGCTGGTACTGGGGCTGAAAACTTCCAGCACTGGGGCCAG24610.1775970776224074No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA24260.1750713166647543No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA24010.1732672016950021No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT23860.17218473271315077No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCT23090.16662805860631397No Hit
GTATTACTGTGCGAGACGTATAGAAGCAGCTGGTACTGGGGCTGAAAACT22910.16532909582809238No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC22650.16345281625955008No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT21830.15753531915876284No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG21790.1572466607636025No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC21360.1541435830156287No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG21180.1528446202374071No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT20950.15118483446523506No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT20870.15060751767491434No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA20420.1473601107293604No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA20360.14692712313661987No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCAA19750.14252508261042446No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG19540.1410096260358326No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA19500.14072096764067224No Hit
ATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATT19450.1403601446467218No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19150.13819520668301916No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT19060.13754572529390835No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC18890.13631892711447685No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT18260.13177255739070132No Hit
GTACATGGGAGCTCTGGGAGACGAGCCCAGCACTGGAAGTCGCCGGTGTT17870.12895813803788786No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17380.12542207269717354No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT17370.12534990809838345No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT16870.12174167815887903No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16770.12102003217097815No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG16560.11950457559638629No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA16520.11921591720122594No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATA16390.11827777741695478No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC16300.117628296027844No Hit
CATATACAGGCAGCAGCACTTTGGTGTTCGGCGGAGGGACCAAGCTGACC16080.11604067485446205No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG16000.11546335806414133No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG15860.11445305368108011No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15740.11358707849559904No Hit
GCTGTGTATTACTGTGCGAGACGTATAGAAGCAGCTGGTACTGGGGCTGA15320.11055616534641534No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA15260.11012317775367479No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA15230.10990668395730453No Hit
GGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGACTTATGATGTC15220.10983451935851446No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT15210.10976235475972436No Hit
TATACAGGCAGCAGCACTTTGGTGTTCGGCGGAGGGACCAAGCTGACCGT15200.10969019016093427No Hit
CTTCTATACGTCTCGCACAGTAATACACAGCCGTGTCCTCAGCTCTCAGG14810.10687577080812083No Hit
CAGTAATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATA14700.10608196022142985No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA14580.10521598503594878No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG14510.10471083284441818No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGCTATGCTATGCACTGGG14510.10471083284441818No Hit
GTATTTATTACTTCCATCATGTGATATAACTGCCACCCACTCCAGCCCCT14380.10377269306014704No Hit
TCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC14300.10319537626982632No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCAAAGTGCTGCTGC14240.1027623886770858No Hit
CTTATGATGTCAGTAAGCGGCCCTCAGGGGTTTCTAATCGCTTCTCTGGC14130.10196857809039482No Hit
ATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATACAGCG14040.10131909670128403No Hit
GCTCATATACAGGCAGCAGCACTTTGGTGTTCGGCGGAGGGACCAAGCTG14010.10110260290491375No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG13950.10066961531217324No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG13920.10045312151580295No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24750.082.192681
GGTATCA8050.071.689131
ATCAACG30800.065.0144353
TCAACGC31250.064.078234
TATCAAC32100.062.6058542
CAACGCA33050.060.804095
AACGCAG33900.059.236736
CGCAGAG38150.052.635718
GCAGAGT42150.047.8113829
TACATGG36700.035.917062
GTACATG37700.035.3988881
ACATGGG38650.034.291313
ATGGGAG13350.033.4511075
TATTGAG19900.031.8514845
CAGAGTA43200.031.8214879
CATGGGA16050.031.4151234
TTATTGA21000.030.8701694
GTACACG15850.030.4470755
GTAATAC26500.030.2159561
CAGCCGT26250.030.16029
Produced by FastQC (version 0.11.5)