FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences776450
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT34700.4469057891686522No Hit
GCCTATATGGAGCTGAGCAGCCTCAGATCTGAGGACACGGCCGTGTATTA25170.3241676862644085No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23150.2981518449352824No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17990.231695537381673No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA17880.23027883315087902No Hit
CCATATAGGCTGTGCTCGTGGATTCGTCCGCGGTAATGGTGACTCTGCCC17280.2225513555283663No Hit
GAGTATTAGCAACTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCC16810.21649816472406463No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA16630.21417992143731085No Hit
ATAATACACGGCCGTGTCCTCAGATCTGAGGCTGCTCAGCTCCATATAGG16420.2114753042694314No Hit
GTAGGAGACAGAGTCTCCATCACTTGCCGGGCAGGTCAGAGTATTAGCAA16340.21044497391976302No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15960.2055509047588383No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT15740.2027174962972503No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC15430.1987249661922854No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGATTCAGTG14510.18687616717109923No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG14170.1824972631850087No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA14120.18185330671646596No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14040.18082297636679762No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13950.1796638547234207No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG13840.17824715049262668No Hit
GAGTTACAGCACCCCCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAAC13680.17618648979329No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC13580.17489857685620452No Hit
ATATGGAGCTGAGCAGCCTCAGATCTGAGGACACGGCCGTGTATTATTGT13520.17412582909395324No Hit
GGTCAGAGTATTAGCAACTATTTAAATTGGTATCAGCAGAAACCAGGGAA13170.16961813381415417No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT13170.16961813381415417No Hit
GTATTATTGTGCGAGAGGGGGTGGTTCGGGAAGAGACTACAACGTCTGGG12870.1657543950028978No Hit
CCCCTGGACAGGGGCTTGAGTGGATGGGAGGGATCATCCCTGTCTTTCCT12770.16446648206581235No Hit
CATATAGGCTGTGCTCGTGGATTCGTCCGCGGTAATGGTGACTCTGCCCT12410.15982999549230473No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12280.15815570867409365No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11970.15416317856912873No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11720.1509433962264151No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG11680.1504282310515809No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG11610.1495266919956211No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCTCCATCACTT11270.14514778800953054No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10930.14076888402344No Hit
CATCAGCAGTGTGCAACCTGAGGATTTTGCAACTTATCACTGTCAACAGA10840.13960976238006312No Hit
GTGTGCAACCTGAGGATTTTGCAACTTATCACTGTCAACAGAGTTACAGC10820.13935217979264602No Hit
CTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAATCT10680.1375491016807264No Hit
CACCATCAGCAGTGTGCAACCTGAGGATTTTGCAACTTATCACTGTCAAC10650.13716272779960076No Hit
CTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGATT10590.13638998003734948No Hit
CAATAATACACGGCCGTGTCCTCAGATCTGAGGCTGCTCAGCTCCATATA10550.1358748148625153No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT10490.135102067100264No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10390.13381415416317854No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG10260.1321398673449675No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10220.1316247021701333No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA10220.1316247021701333No Hit
CCTCTAAAGAAGCCCCTGGGAGCACAGCTCATCACCATGGACTGGACCTG10070.12969283276450513No Hit
GTGTATTATTGTGCGAGAGGGGGTGGTTCGGGAAGAGACTACAACGTCTG10010.12892008500225385No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA9950.12814733724000257No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG9770.12582909395324876No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9570.12325326807907785No Hit
CCATCAGCAGTGTGCAACCTGAGGATTTTGCAACTTATCACTGTCAACAG9460.12183656384828385No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC9180.11823040762444458No Hit
GTATTAGCAACTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCT9130.11758645115590187No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG9110.11732886856848476No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA9060.11668491209994206No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8890.11449546010689678No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8880.11436666881318822No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG8840.11385150363835404No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGGACTGGCT8660.11153326035160023No Hit
ATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGATTCAGTGGCAGT8560.11024534741451478No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG8490.10934380835855496No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG8440.10869985189001223No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG8420.10844226930259515No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA8410.1083134780088866No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG8140.10483611307875587No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA8110.10444973919763025No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC7920.10200270461716789No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC7900.10174512202975078No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT7870.10135874814862515No Hit
GTCTGCATCTGTAGGAGACAGAGTCTCCATCACTTGCCGGGCAGGTCAGA7770.1000708352115397No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14150.079.5299761
TCAACGC16750.066.3065644
ATCAACG17100.064.9536063
TATCAAC17650.063.3381922
CAACGCA17550.063.2799645
AACGCAG18250.060.762636
CGCAGAG21700.051.089058
ACGCAGA24400.045.4357537
GAATACG1601.0822987E-940.493889
CAGCGCT4700.038.2920849
ATACCGT800.002105587936.0038346
GCAGCGC5300.035.3154148
GTACATG21150.034.4487721
TACATGG21850.033.0086062
ACATGGG21700.032.5720443
CATGGGA11600.032.3292734
GCATAGG900.003717805532.0612341
GTACACG8950.030.6184485
GCAGAGT36750.030.3627599
GTATGTA10850.029.2540261
Produced by FastQC (version 0.11.5)