FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences776450
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT32140.4139352179792646No Hit
GCCTATATGGAGCTGAGCAGCCTCAGATCTGAGGACACGGCCGTGTATTA23720.3054929486766695No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18780.24187004958464808No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA17720.22821817245154227No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17340.22332410329061755No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16960.21843003412969283No Hit
CCATATAGGCTGTGCTCGTGGATTCGTCCGCGGTAATGGTGACTCTGCCC16250.20928585227638613No Hit
ATAATACACGGCCGTGTCCTCAGATCTGAGGCTGCTCAGCTCCATATAGG15600.20091441818533068No Hit
GAGTATTAGCAACTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCC15050.19383089703136067No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA14800.19061111468864705No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT14390.1853306716465967No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA13610.17528495073733014No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC13510.1739970378002447No Hit
GTAGGAGACAGAGTCTCCATCACTTGCCGGGCAGGTCAGAGTATTAGCAA13340.17180758580719943No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13320.17155000321978234No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13190.16987571640157126No Hit
GAGTTACAGCACCCCCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAAC13160.16948934252044562No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGATTCAGTG13010.16755747311481745No Hit
GTATTATTGTGCGAGAGGGGGTGGTTCGGGAAGAGACTACAACGTCTGGG12260.15789812608667653No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG12170.15673900444329963No Hit
GGTCAGAGTATTAGCAACTATTTAAATTGGTATCAGCAGAAACCAGGGAA11930.15364801339429454No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT11900.15326163951316893No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC11830.15236010045720907No Hit
ATATGGAGCTGAGCAGCCTCAGATCTGAGGACACGGCCGTGTATTATTGT11610.1495266919956211No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG11580.14914031811449546No Hit
CATATAGGCTGTGCTCGTGGATTCGTCCGCGGTAATGGTGACTCTGCCCT11430.1472084487088673No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11240.14476141412840493No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG10950.1410264666108571No Hit
CTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAATCT10880.1401249275548973No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10630.13690514521218367No Hit
CAATAATACACGGCCGTGTCCTCAGATCTGAGGCTGCTCAGCTCCATATA10480.13497327580655546No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCTCCATCACTT10370.13355657157576148No Hit
CCCCTGGACAGGGGCTTGAGTGGATGGGAGGGATCATCCCTGTCTTTCCT10250.13201107605125892No Hit
GTGTATTATTGTGCGAGAGGGGGTGGTTCGGGAAGAGACTACAACGTCTG10220.1316247021701333No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA10140.13059437182046493No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG10100.13007920664563075No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10090.1299504153519222No Hit
CTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGATT9930.12788975465258548No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9880.12724579818404275No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA9850.12685942430291713No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG9560.1231244767853693No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9520.12260931161053512No Hit
CATCAGCAGTGTGCAACCTGAGGATTTTGCAACTTATCACTGTCAACAGA9510.12248052031682659No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG9390.12093502479232404No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT9350.12041985961748986No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT9280.11951832056153004No Hit
GTATTAGCAACTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCT9220.11874557279927876No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA9190.11835919891815314No Hit
CACCATCAGCAGTGTGCAACCTGAGGATTTTGCAACTTATCACTGTCAAC9040.11642732951252495No Hit
CCTCTAAAGAAGCCCCTGGGAGCACAGCTCATCACCATGGACTGGACCTG8880.11436666881318822No Hit
GTGTGCAACCTGAGGATTTTGCAACTTATCACTGTCAACAGAGTTACAGC8830.11372271234464551No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC8800.11333633846351986No Hit
CCATCAGCAGTGTGCAACCTGAGGATTTTGCAACTTATCACTGTCAACAG8730.11243479940756004No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG8640.11127567776418315No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT8640.11127567776418315No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG8620.11101809517676604No Hit
GTCCTGGTGCACAACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT8590.11063172129564042No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG8440.10869985189001223No Hit
ATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGATTCAGTGGCAGT8330.10728314765921824No Hit
GTCCAGGGGCCTGTCGCACCCAGCTGATAACATTGCTGCTGAAGGTGCCT8230.10599523472213279No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG8010.10316182626054478No Hit
GTCTGCATCTGTAGGAGACAGAGTCTCCATCACTTGCCGGGCAGGTCAGA7980.10277545237941915No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG7970.10264666108571059No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8800.063.771841
ATCAACG10200.056.3931543
TCAACGC10350.054.1899644
TATCAAC11000.052.3019682
TATATCG450.0099618247.931095
CAACGCA12200.045.96675
GAATACG1751.8189894E-1245.250589
CAGCGCT4700.042.8873989
AACGCAG13300.042.1513446
CGCAGAG13500.042.1272288
GTACATG19900.039.7700921
TACATGG20150.038.188222
GGCAGCG5300.036.673937
ACATGGG20800.036.6420023
GTGCTAG2302.910383E-1134.409781
GCAGCGC6200.033.672548
ACGCAGA17200.032.646437
GTATAGG1153.306434E-431.2816161
CATGGGG12350.030.858414
GCCTATA14050.030.7250041
Produced by FastQC (version 0.11.5)