FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281437

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281437
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences867836
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCAGGTCTCAGGCTGTTCATTTGCAGATACA35190.40549136011873205No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT30700.353753474158712No Hit
GTGATGGAAACACCTATTTGAATTGGTTTCAGCAGAGGCCAGGCCAATCT28080.3235634382533105No Hit
GTGTATTACTGTGCGGCCCTCTTTAATTACGATATTTTGACTGGTTCCGA28020.32287206338524793No Hit
GCTATGGACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGC25020.28830331998211645No Hit
TTTTAGAGGTGTCCAGTGTCAGGTGCAACTGGTGGAGTCTGGGGGAGGCG23010.2651422619020184No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21100.24313349526869132No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT18600.2143262090994151No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC18010.2075276895634659No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA18000.20741246041878883No Hit
GTATTACTGTGCGGCCCTCTTTAATTACGATATTTTGACTGGTTCCGAAA17990.2072972312741117No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17170.1978484414105891No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC16640.19174129674270254No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16150.1860950686535244No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA15920.183444798325951No Hit
CAGTAATACACAGCCGTGTCCTCAGGTCTCAGGCTGTTCATTTGCAGATA14670.16904115524131286No Hit
TTATTACTGCATGCAAGGCACACACCGGCCTCCCACCTTCGGCCAAGGGA14240.16408630202019736No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA13530.15590503274812292No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCT13500.1555593453140916No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG13460.15509842873538318No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12820.1477237634760485No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12750.14691715946330874No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG12330.14207753538687032No Hit
CCTTCAATACATATGCTATGGACTGGGTCCGCCAGGCTCCAGGCAAGGGG11940.13758359874446324No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC11810.13608561986366088No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT11630.134011495259473No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA11590.13355057868076456No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG11560.13320489124673326No Hit
TTTATAGGGTCTCTAACCGGGACTCTGGGGTCCCAGACAGATTCAGCGGC11540.13297443295737904No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT11300.13020893348512855No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10990.12663683000013828No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10890.12548453855336725No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC10730.12364087223853354No Hit
GGGCTGGAGTGGGTGGCACTTATCTCATATGATGGAAGCAATACATTCTA10500.12099060191096014No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10190.1174184984259699No Hit
ATTCTACGCAGACTCACTGAAGGACCGATTCACCATCTCCAGAGACAATT10110.11649666526855305No Hit
GTCCAGTGTCAGGTGCAACTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCC10110.11649666526855305No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10090.11626620697919884No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAATACATATGCTATGGACTGGG9810.11303979092823989No Hit
GTCTCCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCT9770.11257887434953148No Hit
GTCTCTAACCGGGACTCTGGGGTCCCAGACAGATTCAGCGGCAGTGGGTC9580.11038952060066648No Hit
ATACACAGCCGTGTCCTCAGGTCTCAGGCTGTTCATTTGCAGATACAGGG9580.11038952060066648No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA9570.11027429145598938No Hit
GCCTGAGACCTGAGGACACGGCTGTGTATTACTGTGCGGCCCTCTTTAAT9560.11015906231131227No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA9550.11004383316663517No Hit
AATTTATAGGGTCTCTAACCGGGACTCTGGGGTCCCAGACAGATTCAGCG9530.10981337487728096No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG9510.10958291658792675No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACACTGAG9280.10693264626035334No Hit
TTCCATCACTGTGTACGAGGCTTTGACTAGACCTGCAGGAGATGGAGGCC9020.10393668849874863No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA8960.103245313630686No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG8900.10255393876262336No Hit
CTGAAGGACCGATTCACCATCTCCAGAGACAATTCCAAGAACACCCTGTA8900.10255393876262336No Hit
GTACATGGGAGCTCTGGGAGACGAGCCCAGCACTGGAAGTCGCCGGTGTT8860.10209302218391492No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC8730.10059504330311257No Hit
GTAATAAACCCCAATATCCTCAGCCTCCACCCTGCTGATTCTCAGTGTGA8700.10024935586908125No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)