FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281438

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281438
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences867836
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCAGGTCTCAGGCTGTTCATTTGCAGATACA32730.3771449905281643No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT27350.31515171069188186No Hit
GTGTATTACTGTGCGGCCCTCTTTAATTACGATATTTTGACTGGTTCCGA27320.31480602325785056No Hit
GTGATGGAAACACCTATTTGAATTGGTTTCAGCAGAGGCCAGGCCAATCT24670.28427029991841773No Hit
GCTATGGACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGC21300.2454380781622334No Hit
TTTTAGAGGTGTCCAGTGTCAGGTGCAACTGGTGGAGTCTGGGGGAGGCG21190.24417055757078526No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT19210.22135518692471848No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18290.21075410561442487No Hit
GTATTACTGTGCGGCCCTCTTTAATTACGATATTTTGACTGGTTCCGAAA18160.20925612673362248No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT16160.1862102977982015No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15580.17952700740692942No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC15110.17411123760710548No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA14840.17100005070082366No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14690.16927161353066708No Hit
CAGTAATACACAGCCGTGTCCTCAGGTCTCAGGCTGTTCATTTGCAGATA14430.16627565576906236No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC14150.16304923971810342No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC11920.13735314045510902No Hit
TTATTACTGCATGCAAGGCACACACCGGCCTCCCACCTTCGGCCAAGGGA11850.1365465364423693No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA11700.13481809927221272No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG11630.134011495259473No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG11570.13332012039141033No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT11310.13032416262980562No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCT11250.129632787761743No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11040.1272129757235238No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT10960.12629114256610696No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA10940.12606068427675274No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10850.12502362197465883No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT10800.12444747625127328No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA10420.12006876875354329No Hit
TTTATAGGGTCTCTAACCGGGACTCTGGGGTCCCAGACAGATTCAGCGGC10420.12006876875354329No Hit
CCTTCAATACATATGCTATGGACTGGGTCCGCCAGGCTCCAGGCAAGGGG10380.11960785217483487No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT10250.11810987329403252No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT9720.11200272862614596No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9550.11004383316663517No Hit
GGGCTGGAGTGGGTGGCACTTATCTCATATGATGGAAGCAATACATTCTA9500.10946768744324964No Hit
GTCTCTAACCGGGACTCTGGGGTCCCAGACAGATTCAGCGGCAGTGGGTC9420.1085458542858328No Hit
GTCCAGTGTCAGGTGCAACTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCC9380.10808493770712438No Hit
GTAATAAACCCCAATATCCTCAGCCTCCACCCTGCTGATTCTCAGTGTGA9360.10785447941777018No Hit
ATACACAGCCGTGTCCTCAGGTCTCAGGCTGTTCATTTGCAGATACAGGG9360.10785447941777018No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC9340.10762402112841597No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG9160.10554989652422808No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAATACATATGCTATGGACTGGG9030.10405191764342571No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT8930.10289962619665467No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8860.10209302218391492No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA8850.10197779303923783No Hit
AATTTATAGGGTCTCTAACCGGGACTCTGGGGTCCCAGACAGATTCAGCG8850.10197779303923783No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC8810.10151687646052941No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG8780.10117118902649809No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACACTGAG8780.10117118902649809No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8150.069.734261
ATCAACG9250.060.649773
TCAACGC9500.059.810824
GGTATCA3950.054.6387671
CAACGCA10450.054.367195
TATCAAC11200.050.7323952
AACGCAG11350.050.6429376
CGCAGAG12250.047.02548
GCAGAGT15400.038.341739
GTAATAC19000.038.242341
TACATGG18000.036.7613642
GTACATG18850.035.4934581
ACATGGG18450.035.085083
AATACAC20500.035.0850753
CAGCCGT20000.034.9237029
TAATACA21800.032.9928472
ACACAGC25600.029.4695916
GTGCTAG2054.3208638E-728.0745531
CCGTGTA1050.00796847627.4314829
AACCGTG1358.412798E-426.6679527
Produced by FastQC (version 0.11.5)