FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281439

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281439
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences821934
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT17330.2108441797029932No Hit
ATATATTACTGTACGAGAGGGGGCATGGCTACAACCTACGGCCGCTTTGA15280.18590300437748042No Hit
GTAATATATGGCCGTGTCTTCAGATTTCAGGTTGCGCAGTTCCATGTAGG15250.18553801156783878No Hit
GCCATATATTACTGTACGAGAGGGGGCATGGCTACAACCTACGGCCGCTT13560.16497674995802583No Hit
CATAAGAACCACATTCCTCCTCTAAAGAAGCCCCTGGGAGCACAGCTCAT12830.15609525825674567No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12750.15512194409770128No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC11530.14027890317227418No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11480.13967058182287143No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA10940.1331007112493217No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAGGGTCTCCTGCA10740.13066742585171073No Hit
GTTGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAA10590.12884246180350248No Hit
ATATATGGCCGTGTCTTCAGATTTCAGGTTGCGCAGTTCCATGTAGGCTG10570.12859913326374137No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT9550.11618937773592528No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT9230.11229612109974767No Hit
ACATGGAACTGCGCAACCTGAAATCTGAAGACACGGCCATATATTACTGT9180.11168779975034493No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9130.11107947840094216No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC8960.10901118581297282No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC8930.10864619300333116No Hit
GAGTTACAGTACTCCTTGGACATTCGGCCAGGGGACCAAGGTGGAAATCA8630.10499626490691467No Hit
TTCCAGGACAGACTCACAATTACCGCGGACGAATCCACAAGGACAGCCTA8500.10341462939846753No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA8440.10268464377918421No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8420.10244131523942313No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17800.073.753281
TCAACGC20350.063.713214
ATCAACG20650.062.7875943
CAACGCA20800.062.6811035
GGTATCA6750.059.843121
AACGCAG22050.059.407416
TATCAAC22600.057.3700832
CGCAGAG25200.051.981488
ACGCAGA27100.048.337027
GCAGAGT29000.045.4183049
CAGCGCT5250.035.6444449
GTACATG25100.035.0603371
GCAGCGC5750.033.7966548
ACATGGG26200.031.3418733
GTACCGG1153.299439E-431.2932016
GATTGCG950.00487914430.328982
TAGCGTT950.004898060630.3049956
CGTTTAA950.004898060630.3049959
ATACCGT950.004898060630.3049956
GAATCGT1204.2341187E-429.9893176
Produced by FastQC (version 0.11.5)