FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281440

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281440
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences821934
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT16820.20463930193908514No Hit
GTAATATATGGCCGTGTCTTCAGATTTCAGGTTGCGCAGTTCCATGTAGG15490.18845795404497198No Hit
ATATATTACTGTACGAGAGGGGGCATGGCTACAACCTACGGCCGCTTTGA15210.18505135448831658No Hit
GCCATATATTACTGTACGAGAGGGGGCATGGCTACAACCTACGGCCGCTT13000.15816355084471503No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12100.14721376655546553No Hit
ATATATGGCCGTGTCTTCAGATTTCAGGTTGCGCAGTTCCATGTAGGCTG11650.1417388744108408No Hit
CATAAGAACCACATTCCTCCTCTAAAGAAGCCCCTGGGAGCACAGCTCAT10670.12981577596254687No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC10660.12969411169266631No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAGGGTCTCCTGCA10220.12434088381792212No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA10040.12215092696007221No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT9670.11764934897449186No Hit
GTTGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAA9200.11193112829010603No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC9150.11132280694070326No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA8790.10694289322500346No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT8750.10645623614548128No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8660.10536125771655631No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC8480.1031713008587064No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG10450.063.9884833
GTATCAA10650.063.5277181
TCAACGC10800.061.918554
CAACGCA11500.056.902535
AACGCAG11700.055.9332436
TATCAAC12950.051.635492
CGCAGAG13650.047.4188278
ACGCAGA16000.040.9011887
GTACATG22550.038.9402121
GCAGAGT17400.037.1992539
ACATGGG23200.036.8802723
TACACGG6800.035.957096
GTACACG6850.034.6426775
ACGGGGT7600.033.1203889
GGTATCA4900.032.315551
ACACGGG7650.031.9618557
GTATAGA2809.094947E-1230.8466661
CATGGGG14700.030.3274544
GTATGTA9000.029.589951
TATGTAC8350.029.277072
Produced by FastQC (version 0.11.5)