FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences971835
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCAGCGCTCAGGCTGTTCATTTGCAGATACA42970.4421532461786209No Hit
GTGTATTACTGTGCGAGAGACTATTACGATGTTAGGACTGGTGTAACTCA39360.4050070227970798No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCG29570.304269757726363No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC23970.2466468073284045No Hit
GAGTTACACCAGTCCTAACATCGTAATAGTCTCTCGCACAGTAATACACA23130.23800336476871073No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA22390.23038890346612334No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT21800.22431791404919557No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT21440.22061358152361252No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC19710.2028122057756718No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC19520.20085714138716962No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA18150.18676009816481193No Hit
GTGTAACTCAGTCCTACTACTTTGACTATTGGGGTCAGGGAACCCTGGTA18020.18542242253057362No Hit
GTATATGAACTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGA16850.1733833418224287No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG16850.1733833418224287No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC16600.1708108886796627No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC15960.16422540863418172No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGAAACCTATAACTATGTC15910.16371091800562854No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG15740.16196164986854766No Hit
GTTCATATACAGGCAGCAGCGTGGTTTTCGGCGGAGGGACCAAGCTGACC15720.16175585361712638No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC14910.15342110543456453No Hit
GTCCTACTACTTTGACTATTGGGGTCAGGGAACCCTGGTAACCGTCTCCT14730.15156893917177297No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT14690.1511573466689304No Hit
GGTGTAACTCAGTCCTACTACTTTGACTATTGGGGTCAGGGAACCCTGGT14450.14868779165187507No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA14340.147555912269058No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC14260.1467327272633729No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG14160.1457037460062665No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGCAATTATGCCATGCACTGGG13850.14251390410923664No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACGCTGCTGCCT13450.13839797908081103No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA13440.1382950809551004No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT13360.13747189594941528No Hit
GTATTACTGTGCGAGAGACTATTACGATGTTAGGACTGGTGTAACTCAGT13350.13736899782370465No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC13220.1360313221894663No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT12930.13304727654385776No Hit
GGGCTGGAGTGGGTGGCAGCTACATCATATGATGGAGGCAATAAATACTA12820.13191539716104073No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTG12800.13170960090961942No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT12710.13078351777822367No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA12390.12749077775548318No Hit
TCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC12330.12687338900121936No Hit
ATACTACGCAGACTCCGTGGAGGGCCGGTTCACCATCTCCAGAGACAAAG11740.12080239958429156No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA11670.12008211270431708No Hit
CTCCTGCACTGAAACCTATAACTATGTCTCCTGGTACCAACAACACCCAG11630.11967052020147453No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA11500.11833284456723621No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC11400.1173038633101298No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGCAATTATGCC11280.11606908580160213No Hit
GTCCTAACATCGTAATAGTCTCTCGCACAGTAATACACAGCCGTGTCCTC11200.115245900795917No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGTTCATAT11020.11339373453312547No Hit
GTACATGGGAGCTCTGGGAGACGAGCCCAGCACTGGAAGTCGCCGGTGTT10990.11308504015599355No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG10990.11308504015599355No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG10980.11298214203028292No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG10960.11277634577886164No Hit
GTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCC10900.11215895702459779No Hit
CAATAAATACTACGCAGACTCCGTGGAGGGCCGGTTCACCATCTCCAGAG10790.11102707764178077No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA10770.11082128139035949No Hit
ATATGATGGAGGCAATAAATACTACGCAGACTCCGTGGAGGGCCGGTTCA10650.10958650388183178No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10650.10958650388183178No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC10560.10866042075043604No Hit
GCTGTGTATTACTGTGCGAGAGACTATTACGATGTTAGGACTGGTGTAAC10550.10855752262472539No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT10550.10855752262472539No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG10510.10814593012188282No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT10430.10732274511619772No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA10380.10680825448764451No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC10330.10629376385909131No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG10330.10629376385909131No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT10330.10629376385909131No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCAC10280.10577927323053811No Hit
CAGTAATACACAGCCGTGTCCTCAGCGCTCAGGCTGTTCATTTGCAGATA10280.10577927323053811No Hit
ATGTTAGGACTGGTGTAACTCAGTCCTACTACTTTGACTATTGGGGTCAG10270.10567637510482747No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA10190.10485319009914235No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC10190.10485319009914235No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT10160.10454449572201041No Hit
ATACACAGCCGTGTCCTCAGCGCTCAGGCTGTTCATTTGCAGATACAACG10140.10433869947058916No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA10050.1034126163391934No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT10030.1032068200877721No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG9990.10279522758492954No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC9990.10279522758492954No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT9870.10156045007640185No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCT9830.10114885757355929No Hit
CCTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAA9810.10094306132213801No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC9760.10042857069358481No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT9720.10001697819074225No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5650.081.696841
GTATCAA15700.077.6355741
ATCAACG18400.064.257033
TCAACGC18600.063.178494
TATCAAC18900.062.5571062
CAACGCA19200.061.2041635
AACGCAG20500.057.2462236
CGCAGAG23800.049.2960368
GTACGGG605.07379E-448.0819851
GCAGAGT27750.042.019749
AGTGGTG7900.035.590345
GGTGGGT7600.035.042058
GTACATG22050.034.9984551
GCGTTAT850.002812931533.9402281
GGGCAGT7750.033.502291
TACATGG22650.033.420712
ACATGGG23750.032.1763533
CAGTGGT9300.031.0078474
TACACCG950.00485911130.3550515
GTATAAC1453.6167243E-529.8439921
Produced by FastQC (version 0.11.5)